A cell wall recycling shortcut that bypasses peptidoglycan de novo biosynthesis
A cell wall recycling shortcut that bypasses peptidoglycan de novo biosynthesis
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DOI：
10.1038/nchembio.1289
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发表时间：
2013-08-01
影响因子：
14.8
通讯作者：
Mayer, Christoph
中科院分区：
文献类型：
--
作者：
Gisin, Jonathan;Schneider, Alexander;Mayer, Christoph
我们报告了革兰氏阴性菌的补救途径，绕过从头生物合成的UDP N-乙酰胞壁酸（UDP-MurNAc），第一个承诺的肽聚糖前体，从而提供了一个内在的磷霉素耐药的理由。异头糖激酶AmgK和MurNAc α-1-磷酸尿苷酰转移酶MurU，在恶臭假单胞菌中定义了这种新的细胞壁糖再循环途径，其特征在于并工程化到大肠杆菌中，将外部MurNAc直接引导到肽聚糖生物合成。
We report a salvage pathway in Gram-negative bacteria that bypasses de novo biosynthesis of UDP N-acetylmuramic acid (UDP-MurNAc), the first committed peptidoglycan precursor, and thus provides a rationale for intrinsic fosfomycin resistance. The anomeric sugar kinase AmgK and the MurNAc alpha-1-phosphate uridylyl transferase MurU, defining this new cell wall sugar-recycling route in Pseudomonas putida, were characterized and engineered into Escherichia coli, channeling external MurNAc directly to peptidoglycan biosynthesis.