STRUCTURE AND FUNCTION OF E COLI RIBOSOMES, .8. COLD-SENSITIVE MUTANTS DEFECTIVE IN RIBOSOME ASSEMBLY
STRUCTURE AND FUNCTION OF E COLI RIBOSOMES, .8. COLD-SENSITIVE MUTANTS DEFECTIVE IN RIBOSOME ASSEMBLY
复制标题
DOI：
10.1073/pnas.63.2.384
复制
发表时间：
1969-01-01
影响因子：
11.1
通讯作者：
NOMURA, M
中科院分区：
文献类型：
--
作者：
GUTHRIE, C;NASHIMOTO, H;NOMURA, M
分离出在 20°C 下无法生长的大肠杆菌突变体，并对三种此类突变体进行了详细研究。其中两个在低温下50S核糖体生物合成有缺陷，分别积累43S和32S颗粒。第三个突变体在低温下产生的 50S 核糖体数量减少，并积累 21S 粒子。 43S 和 32S 颗粒含有 23SRNA，似乎是 50S 核糖体的前体。 21S粒子含有16SRNA。导致 32S 粒子积累的突变与 spclocus 相关。冷敏感突变体的分离和表征应该为体内核糖体组装的研究提供富有成效的方法。
E. colimutants that fail to grow at 20°C were isolated, and three such mutants were studied in detail. Two of these are defective in 50Sribosome biosynthesis at low temperatures and accumulate 43Sand 32Sparticles, respectively. The third mutant produces reduced amounts of 50Sribosomes and accumulates 21Sparticles at low temperatures. The 43Sand 32Sparticles contain 23SRNA and appear to be precursors of 50Sribosomes. The 21Sparticles contain 16SRNA. The mutation that causes accumulation of 32Sparticles is linked to thespclocus. Isolation and characterization of coldsensitive mutants should provide a fruitful approach to the study ofin vivoribosomal assembly.