Analysis of S-locus and expression of S-alleles of self-compatible rapid-cycling Brassica oleracea 'TO1000DH3'
Analysis of S-locus and expression of S-alleles of self-compatible rapid-cycling Brassica oleracea 'TO1000DH3'
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DOI：
10.1007/s11033-014-3526-6
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发表时间：
2014-10-01
影响因子：
2.8
通讯作者：
Ill-Sup, Nou
中科院分区：
文献类型：
--
作者：
Hee-Jeong, Jung;Uddin, Ahmed Nasar;Ill-Sup, Nou
甘蓝是一种严格自交不亲和（SI）植物，但B循环迅速。‘TO 1000 DH 3’是自交亲和的。菊科自交不亲和性受S基因座的复等位基因控制。目前已报道的S位点基因有3种，分别是S位点糖蛋白（SLG）、S位点受体激酶（SRK）和S位点蛋白11（SP11/SCR），它们都属于I类和II类。在这项研究中，我们研究了快速循环B中SI到SC改变背后的分子机制。olerace 'TO1000DH3'。利用SRK特异性标记，通过基因组DNA PCR和PCR-RFLP分析鉴定I类SRK，发现其为纯合型。I类SRK的克隆和测序显示了一个正常的激酶结构域，没有任何S-结构域/跨膜结构域。此外，S位点测序分析仅显示SLG序列，而没有SP11/SCR。表达分析表明SRK在柱头中不表达，而其他参与SI识别反应的基因（SLG、MLPK、ARC 1、THL）在柱头中表达正常。综上所述，上述结果表明，结构畸变，如SI识别基因的缺失可能是负责快速循环B中SI的分解。甘蓝‘TO 1000 DH 3’。
Brassica oleracea is a strictly self-incompatible (SI) plant, but rapid-cycling B. oleracea 'TO1000DH3' is self-compatible (SC). Self-incompatibility in Brassicaceae is controlled by multiple alleles of the S-locus. Three S-locus genes, S-locus glycoprotein (SLG), S-locus receptor kinase (SRK) and S-locus protein 11 or S-locus cysteine-rich (SP11/SCR), have been reported to date, all of which are classified into class I and II. In this study, we investigated the molecular mechanism behind alterations of SI to SC in rapid-cycling B. olerace 'TO1000DH3'. Class I SRK were identified by genomic DNA PCR and PCR-RFLP analysis using SRK specific markers and found to be homozygous. Cloning and sequencing of class I SRK revealed a normal kinase domain without any S-domain/transmembrane domain. Moreover, S-locus sequencing analysis revealed only an SLG sequence, but no SP11/SCR. Expression analysis showed no SRK expression in the stigma, although other genes involved in the SI recognition reaction (SLG, MLPK, ARC1, THL) were found to have normal expression in the stigma. Taken together, the above results suggest that structural aberrations such as deletion of the SI recognition genes may be responsible for the breakdown of SI in rapid-cycling B. oleracea 'TO1000DH3'.