Detection of Akabane viral antigens in spontaneous lymphohistiocytic encephalomyelitis in cattle
Detection of Akabane viral antigens in spontaneous lymphohistiocytic encephalomyelitis in cattle
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DOI：
10.1177/104063870001200605
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发表时间：
2000-11-01
影响因子：
1.5
通讯作者：
Tateyama, S
中科院分区：
文献类型：
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作者：
Uchida, K;Murakami, T;Tateyama, S
1998年8月至10月期间，对一头5个月大的日本黑公牛小牛和27头1-27天大的小牛进行了检查，这些小牛均表现出神经系统体征。公牛犊表现出呼吸急促、发热、超敏反应和共济失调，并在症状发作后4天处以安乐死。27头小牛主要表现为共济失调，15头有关节弯曲。公牛小牛的组织学检查显示血管周围单核细胞浸润，弥漫性多灶性神经胶质增生，以及脑和脊髓中的神经元坏死。在5例中发现了多个软化灶，相比之下，在10月份尸检的15头小牛中，炎性变化较少，但腹角神经元细胞丢失，侧索和腹索中有髓轴突减少。免疫组织化学检查，使用兔抗血清对赤羽病毒株OBE-1发现大量的病毒抗原在退化的神经元和神经胶质细胞的公牛小牛，主要是在脊髓灰质。在27头小牛中的5头中，在肿胀的神经元和少数神经胶质细胞中发现少量病毒抗原。27头小牛中有13头具有较高的赤羽病毒中和抗体滴度，而8月份尸检的大多数小牛中没有显著的抗体滴度。本研究表明，病毒抗原检测是非常有用的诊断赤羽病在5个月大的公牛小牛，怀疑是感染出生后，而它在其他年轻的小牛的有用性有限。
A 5-month-old Japanese black bull calf and twenty-seven 1-27-day-old calves exhibiting neurological signs between August and October 1998 were examined. The bull calf exhibited rapid breathing, fever, hypersensitivity, and ataxia and was euthanized 4 days after the onset of symptoms. The 27 calves primarily exhibited ataxia, and 15 had arthrogryposis. Histological examination of the bull calf revealed perivascular infiltraction by mononuclear cells, diffuse to multifocal gliosis, and neuronal necrosis in the brain and spinal cord. Multiple malacic foci were found in the midbrain in 5 cases, in contrast, in the 15 calves necropsied in October there were fewer inflammatory changes, but there was neuronal cell loss in the ventral horn and a decrease in myelinated axons in the lateral and ventral funiculi. Immunohistochemical examination using a rabbit antiserum against Akabane virus strain OBE-1 revealed a large amount of viral antigen in the degenerating neurons and glial cells of the bull calf, mainly in the spinal gray matter. Small amounts of viral antigen in swollen neurons and a few glial cells were found in 5 of 27 calves. Thirteen of the 27 calves had high neutralization antibody titers against the Akabane virus, whereas there was no significant antibody titer in most of the calves necropsied during August. The present study revealed that viral antigen detection was very useful for the diagnosis of Akabane diseases in the 5-month-old bull calf that was suspected to be infected postnatally, while it had limited usefulness in the other young calves.