Control of morphogenetic cell movements in the early zebrafish myotome
Control of morphogenetic cell movements in the early zebrafish myotome
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DOI：
10.1016/j.ydbio.2007.06.008
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发表时间：
2007-09-15
影响因子：
2.7
通讯作者：
Amacher, Sharon L.
中科院分区：
文献类型：
--
作者：
Daggett, David F.;Domingo, Cannen R.;Amacher, Sharon L.
随着脊椎动物肌节的产生，成肌前体细胞在分化为定位正确的胚胎肌肉纤维时经历了广泛和协调的运动。在斑马鱼中，与脊索相邻的“前轴”细胞是第一个被指定的肌肉前体。这些细胞在最初分化为表达慢收缩肌球蛋白的肌肉纤维后，已被证明经历了显著的径向迁移，穿过外侧体节，分布在成熟肌节的慢收缩肌肉的浅层。在这里，我们描述了一组较早的近轴细胞行为；在迁移事件之前，从大约4×5立方体细胞阵列到1×20延长细胞堆叠的转变。我们发现，近轴细胞在经历这种重排时，会表现出一系列非常典型的行为。此外，我们发现肌动蛋白调节分子Cap1在近轴细胞中特异表达，是这些行为发展所必需的。Cap1对细胞顶端收缩步骤的要求使人想起果蝇发育顶端收缩过程中对Cap的类似要求，这表明对许多发育过程至关重要的细胞生物学事件的基因功能是保守的。(C)2007 Elsevier Inc.保留所有权利。
As the vertebrate myotome is generated, myogenic precursor cells undergo extensive and coordinated movements as they differentiate into properly positioned embryonic muscle fibers. In the zebrafish, the "adaxial" cells adjacent to the notochord are the first muscle precursors to be specified. After initially differentiating into slow-twitch myosin-expressing muscle fibers, these cells have been shown to undergo a remarkable radial migration through the lateral somite, to populate the superficial layer of slow-twitch muscle of the mature myotome. Here we characterize an earlier set of adaxial cell behaviors; the transition from a roughly 4 x 5 array of cuboidal cells to a 1 x 20 stack of elongated cells, prior to the migration event. We find that adaxial cells display a highly stereotypical series of behaviors as they undergo this rearrangement. Furthermore, we show that the actin regulatory molecule, Cap1, is specifically expressed in adaxial cells and is required for the progression of these behaviors. The requirement of Cap1 for a cellular apical constriction step is reminiscent of similar requirements of Cap during apical constriction in Drosophila development, suggesting a conservation of gene function for a cell biological event critical to many developmental processes. (C) 2007 Elsevier Inc. All rights reserved.