Allelic differentiations and effects of the Rf3 and Rf4 genes on fertility restoration in rice with wild abortive cytoplasmic male sterility
Allelic differentiations and effects of the Rf3 and Rf4 genes on fertility restoration in rice with wild abortive cytoplasmic male sterility
复制标题
Rf3和Rf4基因的等位基因分化及其对野败型细胞质雄性不育水稻育性恢复的影响
DOI：
10.1007/s10535-012-0294-9
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发表时间：
2013-06-01
影响因子：
1.5
通讯作者：
Zhang, G. -Q.
中科院分区：
文献类型：
--
作者：
Cai, J.;Liao, Q. P.;Zhang, G. -Q.
为了揭示1号染色体（Rf 3）和10号染色体（Rf 4）上两个育性恢复基因（Rf）的等位基因分化，分别用15个Rf 3和18个Rf 4单片段代换系（SSSL）与野败型细胞质雄性不育系博白A（BbA）杂交。根据F1代花粉和种子的育性，将Rf 3和Rf 4基因分为4个等位基因，即Rf 3的Rf 3 -1、Rf 3 -2、Rf 3 -3和Rf 3 -4，Rf 4的Rf 4 -1、Rf 4 -2、Rf 4 -3和Rf 4 -4。在33个SSSL中，一个携带Rf 3 - 4 Rf 3 -4/Rf 4 - 4 Rf 4 -4基因型的SSSL W23-19-06-06-11对BbA的恢复力最强。为研究Rf 3和Rf 4对WA CMS的遗传效应，以W23 - 19 - 06 - 06 - 11为母本，BbA为父本，通过回交和分子标记辅助选择，获得1个具有W23 - 19-06 - 06 - 11遗传背景的BC 3F 2群体。在BC 3F 2群体中，选择携带Rf 3Rf 3/Rf 4 Rf 4、Rf 3Rf 3/rf 4 rf 4和rf 3rf 3/Rf 4 Rf 4基因型的植物，并评估它们的花粉和小穗育性表型。结果表明，在SSSL W23-19-06-06-11的遗传背景下，Rf 4的效应略大于Rf 3，对WA-CMS系统的效应为加性效应。这些研究将有助于在杂交水稻育种中通过分子标记辅助选择将Rf基因导入适应性品种。
To reveal the allelic differentiations at the two genes for fertility restoration (Rf) on chromosomes 1 (Rf3) and 10 (Rf4), 15 chromosome single segment substitution lines (SSSLs) with the Rf3 locus and 18 SSSLs with the Rf4 locus were crossed with Bobai A (BbA), a cytoplasmic male sterility line with wild abortive type of cytoplasm (WA-CMS), respectively. Based on the pollen and seed fertility of the F-1 hybrids, the Rf3 and Rf4 genes were each classified into four alleles, namely Rf3-1, Rf3-2, Rf3-3, and Rf3-4 for Rf3, and Rf4-1, Rf4-2, Rf4-3, and Rf4-4 for Rf4. Out of the 33 SSSLs, an SSSL W23-19-06-06-11 carrying the genotype Rf3-4Rf3-4/Rf4-4Rf4-4 possessed the strongest restoring ability for BbA. To determine the genetic effects of Rf3 and Rf4 for WA-CMS, one BC3F2 population possessing the genetic background of W23-19-06-06-11 was generated from the cross between W23-19-06-06-11 and BbA by backcrossing and marker-assisted selection. In the BC3F2 population, the plants carrying the Rf3Rf3/Rf4Rf4, Rf3Rf3/rf4rf4, and rf3rf3/Rf4Rf4 genotypes were selected and their phenotyping for pollen and spikelet fertility were evaluated. The result showed that under the genetic background of SSSL W23-19-06-06-11, the effect of Rf4 appeared to be slightly larger than that of Rf3 and their effects were additive for WA-CMS system. These studies will lead to the transfer of Rf genes into adapted cultivars through marker-assisted selection in active hybrid rice breeding programs.