Increased Accumulation of Protein-Bound Nε-(Carboxymethyl)lysine in Tissues of Healthy Rats after Chronic Oral Nε-(Carboxymethyl)lysine
Increased Accumulation of Protein-Bound Nε-(Carboxymethyl)lysine in Tissues of Healthy Rats after Chronic Oral Nε-(Carboxymethyl)lysine
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DOI：
10.1021/jf505063t
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发表时间：
2015-02-11
影响因子：
6.1
通讯作者：
Chen, Jie
中科院分区：
文献类型：
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作者：
Li, Mei;Zeng, Maomao;Chen, Jie
近年来，与晚期糖基化终产物（AGEs）相关的慢性疾病越来越受到人们的关注。由于饮食是AGEs的重要外源性来源，本研究旨在研究健康SpragueDawley大鼠长期口服纯Ne-（羧甲基）赖氨酸（CML）（一种主要的AGE）60 mg kg（-1）/d的影响。给药12周后，肾、心、肝、肺、脾和胰腺中蛋白结合CML的水平分别增加至202 +/- 17、167 +/-47、217 +/- 44、107 +/- 4、144 +/- 23和33 +/- 7 μ g/g干物质，与对照值98 +/- 1、90 +/- 15、140 +/- 42、76 +/- 18、115 +/- 15和30 +/- 4 μ g/g干物质相比。肾脏、心脏、肝脏和肺的差异显著（p < 0.05），而脾脏和胰腺中未见显著增加。此外，血清血尿素氮（BUN）、肌酸酐（CREA）、丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）值显着升高（p < 0.05），这是肾脏和肝脏功能受损的证据。此外，大鼠空腹血糖（FBG）水平保持在正常范围内，表明慢性摄入CML不会促进血糖升高。这些结果清楚地表明，富含CML的饮食可能是人类的潜在健康风险，特别是在肾脏和肝脏功能方面。
In recent years, chronic diseases related to advanced glycation end products (AGEs) have attracted more attention. Because diet is an important exogenous source of AGEs, this study aimed to investigate the effects of chronic oral administration of pure Ne-(carboxymethyl)lysine (CML) (a major AGE) at 60 mg kg(-1) per day on healthy SpragueDawley rats. After administration for 12 weeks, the levels of protein-bound CML were increased to 202 +/- 17, 167 +/- 47, 217 +/- 44, 107 +/- 4, 144 +/- 23, and 33 +/- 7 mu g/g dry matter in the kidneys, heart, liver, lungs, spleen, and pancreas, respectively, in comparison with control values of 98 +/- 1, 90 +/- 15, 140 +/- 42, 76 +/- 18, 115 +/- 15, and 30 +/- 4 mu g/g dry matter. The difference was significant (p < 0.05) for the kidneys, heart, liver, and lungs, whereas no significant increase was seen in the spleen and pancreas. Furthermore, serum blood urea nitrogen (BUN), creatinine (CREA), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) values increased significantly (p < 0.05), as evidence of impaired kidney and liver function. Additionally, the rats fasting blood glucose (FBG) levels remained within the normal range, indicating that chronic intake of CML does not promote a rise in blood glucose. These results clearly indicate that a CML-rich diet might be a potential health risk in humans, particularly with respect to kidney and liver function.