Catalytic and mechanical cycles in F‐ATP synthases
Catalytic and mechanical cycles in F‐ATP synthases
复制标题
F-ATP 合酶的催化和机械循环
作者：
P. Dimroth;Christoph von Ballmoos;T. Meier
周期在各级组织的细胞生命中具有深远的作用。众所周知，细胞代谢的循环包括三羧酸循环和尿素循环，在这两个循环中，特定的载体底物经历一系列的化学转化，并在结束时再生。其他例子包括辅因子的相互转化，如NADH或ATP，它们在细胞中以有限的量存在，必须有效地循环才能继续新陈代谢。每个活细胞都会快速地将ATP转化为ADP，以满足各种能量需求，并在一个耗能的过程中有效地从ADP中再生出ATP。三磷酸腺苷循环的周转率令人印象深刻；一个人每天使用的三磷酸腺苷相当于体重。酶执行催化反应循环，在这些循环中，它们在被转换回原始状态之前经历几次化学和物理转换。普遍存在的F1Fo-ATP合成酶不仅具有重要的生物学意义，而且由于其独特的旋转机制而备受关注。在这里，我们给出了膜包埋的Fo区的概述，特别是关于迟钝伊氏杆菌c环的最新晶体结构，并总结了目前关于c环旋转产生机制的假设。
Cycles have a profound role in cellular life at all levels of organization. Well‐known cycles in cell metabolism include the tricarboxylic acid and the urea cycle, in which a specific carrier substrate undergoes a sequence of chemical transformations and is regenerated at the end. Other examples include the interconversions of cofactors, such as NADH or ATP, which are present in the cell in limiting amounts and have to be recycled effectively for metabolism to continue. Every living cell performs a rapid turnover of ATP to ADP to fulfil various energetic demands and effectively regenerates the ATP from ADP in an energy‐consuming process. The turnover of the ATP cycle is impressive; a human uses about its body weight in ATP per day. Enzymes perform catalytic reaction cycles in which they undergo several chemical and physical transformations before they are converted back to their original states. The ubiquitous F1Fo ATP synthase is of particular interest not only because of its biological importance, but also owing to its unique rotational mechanism. Here, we give an overview of the membrane‐embedded Fo sector, particularly with respect to the recent crystal structure of the c ring from Ilyobacter tartaricus, and summarize current hypotheses for the mechanism by which rotation of the c ring is generated.
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通过半胱氨酸取代蛋白的化学标记和蛋白水解测定，大肠杆菌 ATP 合酶 a 亚基中的螺旋堆积。
影响因子：
2.9
作者：
Zhang,Di;Vik,StevenB
通讯作者：
Vik,StevenB
DOI：
10.1016/s0021-9258(18)43822-7
发表时间：
1994-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Steven B. VikS;B. Antonio
通讯作者：
Steven B. VikS;B. Antonio
全功能 F1Fo ATP 合酶中 c 亚基寡聚物的旋转。
DOI：
10.1073/pnas.98.3.898
发表时间：
2001
影响因子：
11.1
作者：
Tsunoda,SP;Aggeler,R;Yoshida,M;Capaldi,RA
通讯作者：
Capaldi,RA
影响因子：
3.4
作者：
Jianhua Xing;Hongyun Wang;Christoph von Ballmoos;P. Dimroth;G. Oster
通讯作者：
Jianhua Xing;Hongyun Wang;Christoph von Ballmoos;P. Dimroth;G. Oster
影响因子：
2.9
作者：
Girvin, ME;Rastogi, VK;Fillingame, RH
通讯作者：
Fillingame, RH