Terrisporobacter petrolearius sp nov., isolated from an oilfield petroleum reservoir
Terrisporobacter petrolearius sp nov., isolated from an oilfield petroleum reservoir
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DOI：
10.1099/ijsem.0.000450
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发表时间：
2015-10-01
影响因子：
2.8
通讯作者：
Ruan, Zhiyong
中科院分区：
文献类型：
--
作者：
Deng, Yu;Guo, Xiang;Ruan, Zhiyong
从胜利油田某油藏富集样品中分离到一株革兰氏染色阳性、产芽孢、严格厌氧的细菌，命名为LAMOA 37（T）。菌株LAMOA 37（T）的细胞呈杆状，靠周生鞭毛运动。最适生长温度为40 ℃，最适生长pH为7.0-7.5。该菌株生长不需要NaCl，但耐受高达3%（w/v）的NaCl。菌株LAMOA 37（T）能够利用葡萄糖、果糖、麦芽糖、木糖、山梨糖醇、纤维二糖、蜜二糖和三糖作为唯一碳源。亚硫酸盐用作电子受体。葡萄糖发酵的主要产物是乙酸和CO2。主要脂肪酸为C-16：0（23.6%）。主要极性脂质谱由五种糖脂、六种磷脂和两种脂质组成。未检测到甲基萘醌。通过T-m方法测定的基因组DNA G+C含量为27.1 +/-0.2mol%。16 S rRNA基因序列分析表明，该分离物是Terrisporphinus属的成员，并且与Terrisporphinus glycolicus JCM 1401（T）和Terrisporphinus mayombei DSM 6539（T）最接近，两者的16 S rRNA基因序列相似性为98.3%。菌株LAMOA 37（T）与糖梗孢霉和马永贝梗孢霉模式菌株之间的DNA DNA杂交值分别为45.6 +/-0.3%和38.3 +/-0.4%。根据表型、系统发育和化学分类学特征，菌株LAMOA 37（T）被认为是土孢木霉属的一个新种，并建议将其命名为土孢木霉petrolearius sp. nov.。模式菌株为LAMOA 37（T）（=ACCC 00740（T）=JCM 19845（T））。
A Gram-staining-positive, spore-forming, strictly anaerobic bacterium, designated strain LAMOA37(T), was isolated from enrichment samples collected from a petroleum reservoir in Shengli oilfield. Cells of strain LAMOA37(T) were rod-shaped and motile by peritrichous flagella. The optimal temperature and pH for growth were 40 degrees C and 7.0-7.5, respectively. The strain did not require NaCl for growth but tolerated up to 3 % (w/v) NaCl. Strain LAMOA37(T) was able to utilize glucose, fructose, maltose, xylose, sorbitol, cellobiose, melibiose and melezitose as sole carbon sources. Sulfite was used as an electron acceptor. The main products of glucose fermentation were acetate and CO2. The predominant fatty acid was C-16 : 0 (23.6 %). The main polar lipid profile comprised of five glycolipids, six phospholipids and two lipids. No menaquinone was detected. The genomic DNA G+C content was 27.1 +/- 0.2 mol% as determined by the T-m method. Analysis of the 16S rRNA gene sequence indicated that the isolate was a member of the genus Terrisporobacter, and was most closely related to Terrisporobacter glycolicus JCM 1401(T) and Terrisporobacter mayombei DSM 6539(T) with 98.3 % 16S rRNA gene sequence similarity to both. DNA DNA hybridization values between strain LAMOA37(T) and type strains of Terrisporobacter glycolicus and Terrisporobacter mayombei were 45.6 +/- 0.3 % and 38.3 +/- 0.4 %, respectively. Based on phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAMOA37(T) is suggested to represent a novel species of the genus Terrisporobacter, for which the name Terrisporobacter petrolearius sp. nov. is proposed. The type strain is LAMOA37(T) (=ACCC 00740(T)=JCM 19845(T)).