Testing the sorption hypothesis in olfaction: a limited role for sniff strength in shaping primary odor representations during behavior.
Testing the sorption hypothesis in olfaction: a limited role for sniff strength in shaping primary odor representations during behavior.
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DOI：
10.1523/jneurosci.4101-12.2013
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发表时间：
2013-01-02
期刊：
影响因子：
--
通讯作者：
Wachowiak M
中科院分区：
文献类型：
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作者：
Cenier T;McGann JP;Tsuno Y;Verhagen JV;Wachowiak M
在行为过程中获得的感觉信息塑造了神经表征，中央处理和感知外部刺激。在哺乳动物中，嗅代表气味采样的基本单位，但嗅如何塑造气味表征仍然知之甚少。也许最早的假设的作用，嗅嗅觉产生的事实，具有不同的物理化学性质的气味剂表现出不同的模式的沉积在整个嗅觉上皮细胞，这些模式是不同的流量的影响。然而，在自然嗅闻过程中，嗅流率是否形成气味表征仍未经测试，动物是否利用气味吸附-气流关系作为主动气味采样策略的一部分仍不清楚。我们使用三重方法在完整的大鼠中测试了这些想法。首先，我们问是否嗅强度形状的气味表示在体内的嗅觉受体神经元（ORN）终端的成像过程中控制的变化，在麻醉大鼠的吸入流量。第二，我们问是否嗅强度形状的气味表征的成像从ORN在清醒的大鼠自然嗅。第三，我们问大鼠是否积极调节嗅觉强度在气味辨别任务。我们发现，虽然人工改变流速可以改变ORN的反应，嗅强度在自然嗅气味表征的影响可以忽略不计，和行为大鼠不调制流速，以提高气味的歧视。这些数据表明，调制嗅强度不足以形成气味表征的一部分，主动气味感知的行为动物的策略。
The acquisition of sensory information during behavior shapes the neural representation, central processing and perception of external stimuli. In mammals, a sniff represents the basic unit of odor sampling, yet how sniffing shapes odor representations remains poorly understood. Perhaps the earliest hypothesis of the role of sniffing in olfaction arises from the fact that odorants with different physicochemical properties exhibit different patterns of deposition across the olfactory epithelium, and that these patterns are differentially affected by flow rate. However, whether sniff flow rates shape odor representations during natural sniffing remains untested, and whether animals make use of odorant sorption - airflow relationships as part of an active odor sampling strategy remains unclear. We tested these ideas in the intact rat using a three-fold approach. First, we asked whether sniff strength shapes odor representations in vivo by imaging from olfactory receptor neuron (ORN) terminals during controlled changes in inhalation flow in the anesthetized rat. Second, we asked whether sniff strength shapes odor representations by imaging from ORNs during natural sniffing in the awake rat. Third, we asked whether rats actively modulate sniff strength during an odor discrimination task. We found that, while artificial changes in flow rate can alter ORN responses, sniff strength has negligible effect on odor representations during natural sniffing, and behaving rats do not modulate flow rate to improve odor discrimination. These data suggest that modulating sniff strength does not shape odor representations sufficiently to be part of a strategy for active odor sensing in the behaving animal.