Theoretical Considerations of Tissue Electroporation With High-Frequency Bipolar Pulses
Theoretical Considerations of Tissue Electroporation With High-Frequency Bipolar Pulses
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DOI：
10.1109/tbme.2010.2102021
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发表时间：
2011-05-01
影响因子：
4.6
通讯作者：
Davalos, Rafael V.
中科院分区：
文献类型：
--
作者：
Arena, Christopher B.;Sano, Michael B.;Davalos, Rafael V.
本研究介绍了高频脉冲电场在组织电穿孔中的应用。通过有限元模型的发展和分析技术的使用，研究了矩形双极脉冲的电穿孔。电场和温度分布以及相关的跨膜电位发展被认为是在一个非均匀的皮肤褶皱几何。结果表明，在质膜充电时间附近的纳秒尺度上切换极性可以大大改善非均质组织的治疗效果。具体来说，500 kHz至1 MHz范围内的高频场最适合穿透上皮层，而不会引起明显的焦耳加热，并导致下层细胞的电穿孔。
This study introduces the use of high-frequency pulsed electric fields for tissue electroporation. Through the development of finite element models and the use of analytical techniques, electroporation with rectangular, bipolar pulses is investigated. The electric field and temperature distribution along with the associated transmembrane potential development are considered in a heterogeneous skin fold geometry. Results indicate that switching polarity on the nanosecond scale near the charging time of plasma membranes can greatly improve treatment outcomes in heterogeneous tissues. Specifically, high-frequency fields ranging from 500 kHz to 1 MHz are best suited to penetrate epithelial layers without inducing significant Joule heating, and cause electroporation in underlying cells.