Two-Photon Absorbing Dyes with Minimal Autofluorescence in Tissue Imaging: Application to in Vivo Imaging of Amyloid-β Plaques with a Negligible Background Signal
Two-Photon Absorbing Dyes with Minimal Autofluorescence in Tissue Imaging: Application to in Vivo Imaging of Amyloid-β Plaques with a Negligible Background Signal
复制标题
DOI：
10.1021/jacs.5b03548
复制
发表时间：
2015-06-03
影响因子：
15
通讯作者：
Ahn, Kyo Han
中科院分区：
文献类型：
--
作者：
Kim, Dokyoung;Moon, Hyunsoo;Ahn, Kyo Han
组织的荧光成像为研究生物系统提供了必要的手段。自发荧光在UV-vis波长的激发下成为组织成像中的严重问题，其中生物分子与荧光团竞争。为了解决这一关键问题，已经公开了一类新型荧光团，其可以在双光子激发条件下在900 nm附近被激发，并在红色波长区域（>= 600 nm）发射。与广泛使用的双光子吸收染料acedan相比，新的pi-扩展偶极染料系统显示了几个有利的特征，包括组织成像中最小的自荧光和显著的溶剂敏感发射行为。作为新染料系统的重要应用，其中一种染料被开发成淀粉样β斑块的荧光探针，淀粉样β斑块是阿尔茨海默病的关键生物标志物。该探针能够在疾病模型小鼠中对淀粉样蛋白-β斑块进行体内成像，背景信号可忽略不计。新的染料系统具有很大的潜力，开发其他类型的双光子荧光探针和标签的组织成像与最小的自发荧光。
Fluorescence imaging of tissues offer an essential means for studying biological systems. Autofluorescence becomes a serious issue in tissue imaging under excitation at UV-vis wavelengths where biological molecules compete with the fluorophore. To address this critical issue, a novel class of fluorophores that can :be excited at, similar to 900 nm under two-photon excitation conditions and emits in the red wavelength region (>= 600 nm) has been disclosed. The new pi-extended dipolar dye system, shows several advantageous features including minimal antofluorescence in tissue imaging and pronounced solvent-sensitive emission behavior, compared with a widely used two-photon absorbing dye, acedan. As an important application of the new dye system, one of the dyes was developed into a fluorescent probe for amyloicl-beta plaques, a key biomarker of Alzheimer's disease. The probe enabled in Vivo imaging of amyloid-beta plaques in a disease-model mouse, with negligible background signal. The new dye system has great potential for the development of other types of two-photon fluorescent probes and tags for imaging of tissues with minimal autofluorescence.