Bioresponsive nanotherapy for preventing dental caries by inhibiting multispecies cariogenic biofilms.
Bioresponsive nanotherapy for preventing dental caries by inhibiting multispecies cariogenic biofilms.
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通过抑制多物种致龋生物膜预防龋齿的生物响应纳米疗法
DOI：
10.1016/j.bioactmat.2021.12.016
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发表时间：
2022-08
影响因子：
18.9
通讯作者：
Du M
中科院分区：
文献类型：
--
作者：
Liu D;Ma X;Ji Y;Chen R;Zhou S;Yao H;Zhang Z;Ye M;Xu Z;Du M
Early childhood caries (ECC) is a public healthcare concern that greatly reduces the quality of life of young children. As a leading factor of ECC, cariogenic biofilms are composed of acidogenic/aciduric pathogens and extracellular polysaccharides (EPSs), creating an acidic and protected microenvironment. Antimicrobial photodynamic therapy (aPDT) is a noninvasive, painless, and efficient therapeutic approach that is suitable for treating ECC. However, due to the hyperfine structure of cariogenic biofilms, most photosensitizers (PSs) could not access and penetrate deeply in biofilms, which dramatically hamper their efficiency in the clinic. Herein, bioresponsive nanoparticle loaded with chlorin e6 (MPP-Ce6) is developed, which largely increases the penetration depth (by over 75%) and retention (by over 100%) of PS in the biofilm compared with free Ce6. Furthermore, MPP-Ce6-mediated aPDT not only kills the bacteria in preformed biofilms but also inhibits multispecies biofilm formation. A rampant caries model is established to mimic ECC in vivo, where the population of cariogenic bacteria is decreased to 10% after MPP-Ce6-mediated aPDT. Importantly, the number and severity of carious lesions are efficiently reduced via Keyes’ scoring and micro-CT analysis. This simple but effective strategy can serve as a promising approach for daily oral hygiene in preventing ECC. A pH-responsive nano-system is developed for biofilm-targeted drug delivery. The nano-system could overcome biological barriers and penetrate deeply in biofilms. This nano-system facilitates aPDT to kill bacteria in deep cariogenic biofilm. This strategy prevents the progression of early childhood caries in a rat model.
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影响因子：
5.8
作者：
Li, Zhiyuan;Pan, Wei;Wang, Yue
通讯作者：
Wang, Yue
影响因子：
4.2
作者：
LINDQUIST, B;EMILSON, CG
通讯作者：
EMILSON, CG
影响因子：
2.2
作者：
Pereira, Cássio Vicente;Pereira, Luciano José;Höfling, José Francisco
通讯作者：
Höfling, José Francisco
影响因子：
16.6
作者：
Deng, H;Li, XL;Li, YD
通讯作者：
Li, YD
影响因子：
17.1
作者：
Hu, Dengfeng;Deng, Yongyan;Ji, Jian
通讯作者：
Ji, Jian