Protein oxidation in human stratum corneum: susceptibility of keratins to oxidation in vitro and presence of a keratin oxidation gradient in vivo.
Protein oxidation in human stratum corneum: susceptibility of keratins to oxidation in vitro and presence of a keratin oxidation gradient in vivo.
复制标题
人角质层中的蛋白质氧化：角蛋白对体外氧化的敏感性以及体内角蛋白氧化梯度的存在。
DOI：
--
复制
发表时间：
1999
影响因子：
6.5
通讯作者：
H. Sies
中科院分区：
文献类型：
--
作者：
J. Thiele;S. N. Hsieh;K. Briviba;H. Sies
角质层位于身体和环境的交界处，因此不断地暴露在有利于氧化的环境中。以前，我们已经证明角质层脂类是臭氧和紫外线A和B暴露引起的氧化应激的靶标。在这里，我们使用了一种免疫印迹技术来检测通过胶带剥离获得的人角质层中的蛋白质氧化。裂解后，用二硝基苯肼衍生化、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法分离、二硝基苯基抗体免疫印迹法检测蛋白质的羰基。角蛋白10通过使用特定抗体和显微测序鉴定，在体外被证明可被紫外线A辐射、次氯酸盐和过氧化苯甲酰氧化。在活体中，健康志愿者前额角质层(n=6)的角蛋白10氧化梯度在角质层下层较低，而向外层的羰基含量较高约3倍。由于蛋白质氧化可能与对蛋白酶的易感性增加有关，这一发现可能对更好地理解脱皮过程是重要的。
The stratum corneum is located at the interface between body and environment and thus is constantly exposed to a pro-oxidative environment. Previously, we have demonstrated that stratum corneum lipids are targets of oxidative stress induced by ozone and by ultraviolet A and B exposure. Here, we employed an immunoblotting technique to detect protein oxidation in human stratum corneum obtained by tape stripping. After lysis, protein carbonyl groups were measured by derivatization with dinitrophenylhydrazine, separation by sodium dodecylsulfate-polyacrylamide gel electrophoresis, and immunoblotting using antibodies against dinitrophenyl groups. Keratin 10, identified by use of specific antibodies and by microsequencing, was demonstrated in vitro to be oxidizable by ultraviolet A irradiation, hypochlorite, and benzoyl peroxide. In vivo, a keratin 10 oxidation gradient with low levels in the lower stratum corneum layers, and about 3-fold higher contents of carbonyl groups towards the outer layers was demonstrated in forehead stratum corneum of healthy volunteers (n = 6). As protein oxidation can be associated with an increased susceptibility to proteases, this finding may be important for better understanding the process of desquamation.
脱屑素的酶活性。
影响因子：
3.7
作者：
Brysk,MM;Bell,T;Brysk,H;Selvanayagam,P;Rajaraman,S
通讯作者：
Rajaraman,S
食管上皮分化过程中基底上层的变化以及随后二硫键稳定的角蛋白同二聚体和异二聚体的形成。
影响因子：
4
作者：
Pang,YY;Schermer,A;Yu,J;Sun,TT
通讯作者：
Sun,TT
影响因子：
4.2
作者：
Ibrahim, W;Lee, US;Chow, CK
通讯作者：
Chow, CK