A novel technique using hydrophilic polymers to promote axonal fusion.
A novel technique using hydrophilic polymers to promote axonal fusion.
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DOI：
10.4103/1673-5374.180724
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发表时间：
2016-04
影响因子：
6.1
通讯作者：
Thayer WP
中科院分区：
文献类型：
--
作者：
Bamba R;Riley DC;Kelm ND;Does MD;Dortch RD;Thayer WP
外伤性周围神经损伤的处理仍然是临床医生相当关注的问题。由于手术技术上的创新很少，而且训练有素的治疗周围神经损伤的专家数量有限，手术干预的结果是不可预测的。由于无法控制神经损伤的病理生理学（即沃勒氏变性），科学家和临床医生只能依靠缓慢而漫长的轴突再生过程（约1毫米/天）。当轴突被切断时，其末梢经历钙介导的质浆封合，这限制了轴突主要修复的能力。聚乙二醇（PEG）与生物工程工艺相结合，克服了轴突融合的无能。PEG轴突融合的机制尚不清楚，但多项研究表明，提供无钙环境对PEG融合过程至关重要。提出的机制是通过去除腋膜周围的水合屏障和降低膜融合所需的活化能来诱导peg诱导的脂质双分子层融合。本文综述了聚乙二醇融合的研究现状，并对未来的研究方向进行了展望。
The management of traumatic peripheral nerve injury remains a considerable concern for clinicians. With minimal innovations in surgical technique and a limited number of specialists trained to treat peripheral nerve injury, outcomes of surgical intervention have been unpredictable. The inability to manipulate the pathophysiology of nerve injury (i.e., Wallerian degeneration) has left scientists and clinicians depending on the slow and lengthy process of axonal regeneration (~1 mm/day). When axons are severed, the endings undergo calcium-mediated plasmalemmal sealing, which limits the ability of the axon to be primarily repaired. Polythethylene glycol (PEG) in combination with a bioengineered process overcomes the inability to fuse axons. The mechanism for PEG axonal fusion is not clearly understood, but multiple studies have shown that a providing a calcium-free environment is essential to the process known as PEG fusion. The proposed mechanism is PEG-induced lipid bilayer fusion by removing the hydration barrier surrounding the axolemma and reducing the activation energy required for membrane fusion to occur. This review highlights PEG fusion, its past and current studies, and future directions in PEG fusion.