Bactericidal effect of photodynamic inactivation against methicillin-resistant and methicillin-susceptible Staphylococcus aureus is strain-dependent
Bactericidal effect of photodynamic inactivation against methicillin-resistant and methicillin-susceptible Staphylococcus aureus is strain-dependent
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DOI：
10.1016/j.jphotobiol.2007.11.002
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发表时间：
2008-01-30
影响因子：
5.4
通讯作者：
Bielawski, Krzysztof P.
中科院分区：
文献类型：
--
作者：
Grinhoic, Mariusz;Szramka, Bozena;Bielawski, Krzysztof P.
金黄色葡萄球菌是引起医院感染的重要病原菌之一。其中一些可能危及生命，特别是在免疫功能低下的患者中，导致菌血症、心内膜炎、败血症或中毒性休克综合征。它们对抗生素的多重耐药性产生了许多治疗问题，因此需要开发一种替代抗生素治疗的方法。光动力学灭活（PDI）可能是一种有效的替代治疗方法，可用于治疗甲氧西林耐药（MRSA）和甲氧西林敏感（MSSA）S。金黄色葡萄球菌本研究的目的是分析PDI对从格但斯克省医院住院患者中分离的40株临床MRSA和40株MSSA临床菌株的杀菌效果。ATCC菌株25904已用作参考。研究了以二精氨酸原卟啉为光敏剂的光动力学灭活作用。据观察，PDI的杀菌作用是菌株依赖性的，范围为0至3 log（10）单位的活菌计数减少。这种现象背后的机制仍然不清楚。然而，已经观察到生物膜产生能力与不同菌株对PDI的响应之间的相关性。(c)2007 Elsevier B.V.保留所有权利。
Staphylococcus aureus is one of the most important etiological factors responsible for nosocomial infections. Some of them may be life-threatening, especially in the case of immuno-compromised patients, causing bacteremia, endocarditis, sepsis or toxic-shock syndrome. Their multiresistance to antibiotics produces many therapeutic problems, and for this reason the development of a method alternative to antibiotic therapy is needed. It seems that photodynamic inactivation (PDI) may be an effective and alternative therapeutic option against both methicillin resistant (MRSA) and methicillin sensitive (MSSA) S. aureus strains. The aim of this study was to analyze the bactericidal effect of the PDI against 40 clinical MRSA and 40 MSSA clinical strains that were isolated from patients hospitalized in the Provincial Hospital in Gdansk. The ATCC strain 25904 has been used as a reference. Photodynamic inactivation by means of protoporphyrin diarginate as a photosensitizer was examined. It was observed that the bactericidal effect of the PDI was strain-dependent and ranged from 0 to 3 log(10)-unit reduction in viable counts. The mechanism underlying such a phenomenon is still not understood. Nevertheless, the correlation between the biofilm production ability and different strains response to PDI has been observed. (c) 2007 Elsevier B.V. All rights reserved.