Distribution of latex beads and retinal pigment epithelial cells along the ventral neural crest pathway.
Distribution of latex beads and retinal pigment epithelial cells along the ventral neural crest pathway.
复制标题
乳胶珠和视网膜色素上皮细胞沿腹侧神经嵴通路的分布。
DOI：
10.1016/0012-1606(82)90007-0
复制
发表时间：
1982
影响因子：
2.7
通讯作者：
Bronner-Fraser,M
中科院分区：
文献类型：
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作者：
Bronner-Fraser,M
神经嵴细胞迁移沿着确定的途径在鸟类胚胎的躯干。先前的研究表明，嵴源性色素细胞在注入腹侧神经嵴通路后向腹侧迁移（M。E. Bronner-Fraser和A. M. Cohen，1980，Develop. Biol.77，130 -141）。在本研究中，乳胶聚苯乙烯珠和视网膜色素上皮（RPE）细胞注射到腹侧通路，以探测环境沿着这一迁移路线。虽然RPE细胞是非运动的，不是来自神经嵴，他们也易位腹侧。因此，独立于主动迁移的因素可能会影响RPE细胞（和内源性嵴细胞）的定位。为了测试这种可能性，将乳胶聚苯乙烯珠注射到腹侧通路上。使用三种类型的珠：（a）未涂覆的乳胶珠;（B）用牛血清白蛋白涂覆的乳胶珠（BSA-珠）;和（c）用纤连蛋白涂覆的乳胶珠（FN-珠）。无涂层和BSA珠分布沿着腹侧通路类似的RPE细胞和内源性嵴细胞。然而，FN-珠保持在植入部位附近，并且不向腹侧移动。结果表明：（1）细胞表面的纤连蛋白等分子可能是一种识别机制，阻止进入腹侧通路;（2）嵴细胞定位可能部分受到胚胎环境赋予的驱动力的影响。
Neural crest cells migrate along defined pathways in the trunk of avian embryos. Previous studies have demonstrated that crest-derived pigment cells migrated ventrally after injection onto the ventral neural crest pathway (M. E. Bronner-Fraser and A. M. Cohen, 1980,Develop. Biol.77,130–141). In the present study, latex polystyrene beads and retinal pigment epithelial (RPE) cells were injected onto the ventral pathway in order to probe the environment along this migratory route. Although the RPE cells are nonmotile and not derived from the neural crest, they also translocated ventrally. Thus, factors independent of active migration may affect the localization of RPE cells (and endogenous crest cells). To test this possibility, latex polystyrene beads were injected onto the ventral pathway. Three types of beads were used: (a) uncoated latex beads; (b) latex beads coated with bovine serum albumin (BSA-beads); and (c) latex beads coated with fibronectin (FN-beads). Uncoated and BSA-beads distributed along the ventral pathway similarly to RPE cells and endogenous crest cells. However, FN-beads remained near the site of implantation and did not move ventrally. The results suggest (1) that molecules like fibronectin on the cell surface might serve as a recognition mechanism that prevents entrance onto the ventral pathway; and (2) that crest cell localization may, in part, be influenced by a driving force imparted by the embryonic environment.