The rice REDUCED CULM NUMBER11 gene controls vegetative growth under low-temperature conditions in paddy fields independent of RCN1/OsABCG5
The rice REDUCED CULM NUMBER11 gene controls vegetative growth under low-temperature conditions in paddy fields independent of RCN1/OsABCG5
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水稻REDUCED CULM NUMBER11基因独立于RCN1/OsABCG5控制稻田低温条件下的营养生长
DOI：
10.1016/j.plantsci.2013.06.011
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发表时间：
2013
期刊：
影响因子：
5.2
通讯作者：
Kiyoaki Kato
中科院分区：
文献类型：
--
作者：
Atsushi Funabiki;Sho Takano;Shuichi Matsuda;Yoshihiko Tokuji;Itsuro Takamure;Kiyoaki Kato
营养生长阶段的低温耐受性是水稻育种的重要目标。通过低温条件下稻田筛选，分离到一个新的减少茎数突变体，命名为减少茎数11（rcn11）。由于thercn11的茎部结构与thercn1非常相似，我们研究了RCN11是否参与了RCN1/ osabcg5相关的营养生长控制。thercn11突变体在RCN1/OsABCG5基因中没有突变，并且rcn11对RCN1/OsABCG5基因的表达没有影响。在cn1突变体中，RCN1/OsABCG5表达上调，表明RCN1/OsABCG5受负反馈调控。RCN11对RCN1/OsABCG5反馈调控的影响的缺失支持了RCN11不参与RCN1/OsABCG5相关的转运系统。遗传等位基因测试和分子定位研究表明，rcn11独立于水稻3号染色体上的rcn11，位于8号染色体上。rcn1 rcn11表型表明rcn11独立于rcn1 /OsABCG5作用于营养生长。幼苗根系发育比较表明，rcn11降低了树冠根数和伸长，而rcn1降低了侧根密度和伸长。因此，rcn11将为低温条件下的营养生长控制提供新的思路。
Low temperature tolerance during vegetative growth is an important objective in rice (Oryza sativaL.) breeding programs. We isolated a novel reduced culm number mutant, designatedreduced culm number11(rcn11), by screening under low-temperature condition in a paddy fields. Since the shoot architecture of thercn11was very similar to that of thercn1, we examined whether RCN11 is involved in RCN1/OsABCG5-associated vegetative growth control. Thercn11mutant has no mutation in the RCN1/OsABCG5 gene andrcn11has no effect on RCN1/OsABCG5 gene expression. In thercn1mutant,RCN1/OsABCG5was upregulated showing thatRCN1/OsABCG5 is controlled by negative feedback regulation. Absence of an effect ofrcn11on RCN1/OsABCG5 feedback regulation supported that RCN11 is not involved in the RCN1/OsABCG5-associated transport system. A genetic allelism test and molecular mapping study showed thatrcn11is independent ofrcn1on rice chromosome 3 and located on chromosome 8. Thercn1 rcn11phenotype suggests thatRCN11acts on vegetative growth independent ofRCN1/OsABCG5. A root development comparison betweenrcn1andrcn11in young seedlings represented thatrcn11reduced crown root number and elongation, whereasrcn1reduced lateral root density and elongation. Thus,rcn11will shed new light on vegetative growth control under low temperature.