Identification of a Biomarker Panel for Early Detection of Lung Cancer Patients
Identification of a Biomarker Panel for Early Detection of Lung Cancer Patients
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DOI：
10.1021/acs.jproteome.9b00287
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发表时间：
2019-09-01
影响因子：
4.4
通讯作者：
Whetton, Anthony D.
中科院分区：
文献类型：
--
作者：
Geary, Bethany;Walker, Michael J.;Whetton, Anthony D.
肺癌是全球癌症相关死亡的最常见原因，其特征在于晚期临床表现（49-53%的患者在IV期诊断），因此预后较差。鉴定早期疾病的生物标志物的一个挑战是在症状出现之前从患者收集样品。我们在iTRAQ等压标记实验中使用在肺肿瘤手术切除期间收集的血液来鉴定从肿瘤流出到肺静脉中的蛋白质。与“健康”肺静脉相比，40种蛋白质被鉴定为在从肿瘤引流的静脉中具有增加的丰度。然后在第二组中评估这些蛋白质标记物，该第二组利用质谱（MS）技术：所有理论碎片离子谱（SWATH）MS的顺序窗口采集。SWATH-MS用于测量取自25名患者的血清样品中的蛋白质
Lung cancer is the most common cause of cancer-related mortality worldwide, characterized by late clinical presentation (49-53% of patients are diagnosed at stage IV) and consequently poor outcomes. One challenge in identifying biomarkers of early disease is the collection of samples from patients prior to symptomatic presentation. We used blood collected during surgical resection of lung tumors in an iTRAQ isobaric tagging experiment to identify proteins effluxing from tumors into pulmonary veins. Forty proteins were identified as having an increased abundance in the vein draining from the tumor compared to "healthy" pulmonary veins. These protein markers were then assessed in a second cohort that utilized the mass spectrometry (MS) technique: Sequential window acquisition of all theoretical fragment ion spectra (SWATH) MS. SWATH-MS was used to measure proteins in serum samples taken from 25 patients