Development and application of a simple loop-mediated isothermal amplification method on rapid detection of Listeria monocytogenes strains
Development and application of a simple loop-mediated isothermal amplification method on rapid detection of Listeria monocytogenes strains
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一种简单的环介导等温扩增方法快速检测单增李斯特菌菌株的开发和应用
DOI：
10.1007/s11033-011-0757-7
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发表时间：
2012-01-01
影响因子：
2.8
通讯作者：
Zhong, Qingping
中科院分区：
文献类型：
--
作者：
Wang, Li;Li, Yue;Zhong, Qingping
建立了一种快速检测食源性李斯特菌的环介导等温扩增（LAMP）方法。单核细胞增生菌株已在本研究中开发和评估。最佳反应条件为65 ℃，反应45 min，检测限为1 pg DNA/管，100 CFU/反应。应用建立的LAMP方法对182株食源性乳杆菌进行检测。LAMP法与PCR法的敏感性分别为96.7%（176/182）和91.2%（166/182），特异性、阳性预测值（PPV）和阴性预测值（NPV）均为100%。
A loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borne L. monocytogenes strains had been developed and evaluated in this study. The optimal reaction condition was 65A degrees C for 45 min, with the detection limit as 1 pg DNA/tube and 100 CFU/reaction. Application of the established LAMP assay was performed on 182 food-borne L. monocytogenes strains using a rapid procedure and easy result confirmation, with the sensitivity of LAMP versus PCR assays as 96.7% (176/182) and 91.2% (166/182), respectively; with 100% specificity, positive predictive value (PPV) and negative predictive value (NPV) for both assays.