Proteomic identification of salivary transferrin as a biomarker for early detection of oral cancer
Proteomic identification of salivary transferrin as a biomarker for early detection of oral cancer
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DOI：
10.1016/j.aca.2010.09.030
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发表时间：
2010-11-29
影响因子：
6.2
通讯作者：
Lin, Cheng-Wen
中科院分区：
文献类型：
--
作者：
Jou, Yu-Jen;Lin, Chia-Der;Lin, Cheng-Wen
口腔癌的五年生存率很低。早期发现口腔癌可以降低与这种疾病相关的死亡率和发病率。唾液可以非侵入性地采样，并且比血液更简单，是口腔癌生物标志物的良好潜在来源。对来自口腔癌患者和对照受试者的唾液进行蛋白质组学分析以鉴定人类早期口腔癌的唾液生物标志物。采用二维凝胶电泳（2DE）和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）分析口腔鳞状细胞癌（OSCC）患者或无OSCC对照者的合并唾液样本的蛋白质谱。通过Western印迹和ELISA测定验证潜在的生物标志物。转铁蛋白水平升高，在口腔鳞癌患者的唾液中使用2DE确定，然后通过MALDI-TOF MS和MALDI-TOF/TOF MS，蛋白质印迹和ELISA证实。口腔鳞癌患者唾液转铁蛋白水平的增加与肿瘤的大小和分期密切相关。受试者工作特征曲线下面积表明，唾液转铁蛋白ELISA对口腔癌的早期诊断具有高度的特异性、敏感性和准确性。我们已经确定唾液转铁蛋白作为检测早期口腔癌的生物标志物。这一发现为开发早期口腔癌的非侵入性诊断测试提供了有希望的基础。皇冠版权所有（C）2010由爱思唯尔B. V.出版保留所有权利。
Oral cancer has a low five-year survival rate. Early detection of oral cancer could reduce the mortality and morbidity associated with this disease. Saliva, which can be sampled non-invasively and is less complex than blood, is a good potential source of oral cancer biomarkers. Proteomic analysis of saliva from oral cancer patients and control subjects was performed to identify salivary biomarkers of early stage oral cancer in humans. The protein profile of pooled salivary samples from patients with oral squamous cell carcinoma (OSCC) or OSCC-free control subjects was analyzed using two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analyses. Potential biomarkers were verified by Western blotting and ELISA assays. Transferrin levels were elevated in the saliva of OSCC patients as determined using 2DE followed by MALDI-TOF MS and confirmed by MALDI-TOF/TOF MS, Western blotting and ELISA. The increase in salivary transferrin levels in OSCC patients strongly correlated with the size and stage of the tumor. The area under the receiver-operating characteristics curves showed that salivary transferrin-based ELISA was highly specific, sensitive and accurate for the early detection of oral cancer. We have identified salivary transferrin as a biomarker for the detection of early stage oral cancer. This finding provides a promising basis for the development of a non-invasive diagnostic test for early stage oral cancer. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.