β-carotene is the intermediate exported from the chloroplast during accumulation of secondary carotenoids in Haematococcus pluvialis
β-carotene is the intermediate exported from the chloroplast during accumulation of secondary carotenoids in Haematococcus pluvialis
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DOI：
10.1023/a:1008183328839
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发表时间：
2001-02-01
影响因子：
3.3
通讯作者：
Hagen, C
中科院分区：
文献类型：
--
作者：
Grünewald, K;Hagen, C
最近对雨生红球藻合成虾青素的早期和晚期酶定位的研究结果表明，虾青素合成的两个隔室，叶绿体(早期合成的位置)和细胞质脂泡依次参与其中。通过二苯胺(DPA)和2-(4-氯苯硫基)-三乙胺(CPTA)抑制剂，阐明了穿过叶绿体被膜进入细胞质的中间体。根据抑制剂处理过程中积累的中间体的数量以及它们在脂泡组分中的存在，我们得出结论，这种转运发生在β-紫罗兰酮环化之后。β-紫罗兰酮类胡萝卜素通过类胡萝卜素结合蛋白从叶绿体向细胞质脂泡运输的非囊泡机制被提出。
Recent findings on the localization in Haematococcus pluvialis of an early and a late enzyme in astaxanthin synthesis have suggested the sequential involvement of two compartments, the chloroplast (site of early synthesis) and the cytoplasmic lipid vesicles. Here the intermediate transported across the chloroplast envelope into the cytoplasm is elucidated by means of the inhibitors diphenylamine (DPA) and 2-(4-chlorophenylthio)-triethylamine (CPTA). Based on the amounts of the intermediates accumulated upon inhibitor treatment and their occurrence in the lipid vesicle fractions, it is concluded that the transport takes place after beta -ionone ring cyclization. A non-vesicle mechanism of beta -ionone carotenoid transport from the chloroplast towards the cytoplasmic lipid vesicles via carotenoid binding proteins is suggested.