Dinucleotide microsatellites from Eucalyptus sieberi:: inheritance, diversity, and improved scoring of single-base differences
Dinucleotide microsatellites from Eucalyptus sieberi:: inheritance, diversity, and improved scoring of single-base differences
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DOI：
10.1139/gen-44-6-1041
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发表时间：
2001-12-01
期刊：
影响因子：
3.1
通讯作者：
Moran, GF
中科院分区：
文献类型：
--
作者：
Glaubitz, JC;Emebiri, LC;Moran, GF
利用8个二核苷酸微卫星标记在西伯利亚桉中进行了扩增。约翰逊（银顶灰），桉树亚属的成员。转移其中六个亚属Symphyomyrtus和孟德尔遗传证明使用全同胞杂交在光叶桉。以100份老龄E. Es 266位点具有较高的固定指数，且显著偏离Hardy-Weinberg平衡基因型比例，表明可能存在无效等位基因。其中两个基因座Es 076和Es 140有许多等位基因，它们在大小上只有一个碱基对的差异，这可能是因为它们侧翼区域的短poly（A）或poly（T）延伸。这两个基因座是迄今为止最多态的，但很难可靠地评分毛细管DNA测序仪。这两个单碱基微卫星位点的评分的可靠性显着提高了内部参考等位基因纳入每个分析的样品。
Eight dinucleotide microsatellites were developed in Eucalyptus sieberi L. Johnson (silvertop ash), a member of the subgenus Eucalyptus. Transfer of six of these to the subgenus Symphyomyrtus and their Mendelian inheritance are demonstrated using a full-sib cross in Eucalyptus nitens. Genetic diversity parameters are presented for the eight loci based on a sample of 100 old-growth E. sieberi trees from a single natural stand. One locus, Es266, had an atypically high fixation index, and significantly deviated from Hardy-Weinberg equilibrium genotypic proportions, indicating the likely presence of null alleles. Two of the loci, Es076 and Es140, had many alleles that differed in size by only a single base pair, possibly because of short poly(A) or poly(T) stretches in their flanking regions. These two loci were by far the most polymorphic, but were difficult to score reliably on a capillary DNA sequencer. Reliability of scoring of these two one-base microsatellite loci was markedly improved by the incorporation of internal reference alleles into each sample analysed.