CRYSTALLINE AND AMORPHOUS INSULIN-ZINC COMPOUNDS WITH PROLONGED ACTION
CRYSTALLINE AND AMORPHOUS INSULIN-ZINC COMPOUNDS WITH PROLONGED ACTION
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DOI：
10.1126/science.116.3015.394
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发表时间：
1952-01-01
期刊：
影响因子：
56.9
通讯作者：
SCHLICHTKRULL, J
中科院分区：
文献类型：
--
作者：
HALLASMOLLER, K;PETERSEN, K;SCHLICHTKRULL, J
Fig. 1.胰岛素（40 u/ml）沉淀区：A，0.01 mol磷酸钠; B，0.01 mol磷酸钠和2 mg锌（氯化物）。1000 u; C，0.01 mol乙酸钠; D，0.01 mol乙酸钠与2 mg锌（以氯化物计）/1000 u; E，0.01 mol磷酸钠与！2 mg锌（以氯化物计）/1000 u和8.5mg鱼精蛋白/1000 u。在磷酸盐缓冲液中pH的函数（将40 μ/ml的胰岛素酸溶液调节至不同的pH值，溶解的胰岛素用比色法测定）。可以看出，所有的胰岛素在血液的pH 7.3下溶解，这大概解释了为什么等电沉淀的胰岛素不具有持续作用。B显示胰岛素在已加入与鱼精蛋白-锌-胰岛素中相同量的锌的缓冲液中的溶解度-2 mg/1000 u。溶解度的时间条件
FIG. 1. Precipitation zone of insulin (40 u/ml) in: A, 0.01 mol sodium phosphate; B, 0.01 mol sodium phosphate with 2 mg zinc (as chloride)./1000 u; C, 0.01 mol sodium acetate; D, 0.01 mol sodium acetate with 2 mg zinc (as chloride)/1000 u; E, 0.01 mol sodium phosphate with! 2 mg zinc (as chloride)/1000 u and 8.5 mg protamilne/1000 u. a function of pH in phosphate buffer (an acid solution of insulin-40 u/nil-is adjusted to different pH values, and the dissolved insulin is deterimiined spectrophotometrically). It can be seen that all the insulin is dissolved at the pH of blood, 7.3, which presumably explains why isoelectrically precipitated insulin does not possess a sustained effect. B shows the solubility of insulin in the samiie buffer, to which has been added the same amount of zinc as is found in protamine-zine-insulin-2 mg/1000u. Time conditions for solubility