Antibacterial steroid alkaloids from the stem bark of Holarrhena pubescens
Antibacterial steroid alkaloids from the stem bark of Holarrhena pubescens
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DOI：
10.1016/s0378-8741(99)00119-1
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发表时间：
1999-12-15
影响因子：
5.4
通讯作者：
Brantner, AH
中科院分区：
文献类型：
--
作者：
Chakraborty, A;Brantner, AH
毛冬青Holarrhena pubescens(syn.抗痢疾杆菌)(L.)墙。用微量肉汤稀释法和纸片扩散法检测了树皮对金黄色葡萄球菌、表皮葡萄球菌、粪链球菌、枯草芽孢杆菌、大肠杆菌和铜绿假单胞菌的抑菌效果。粗甲醇提取物对所有受试菌均有抑制作用。进一步的化学分级表明，其抑菌活性主要与生物碱有关。采用微量稀释法测定其粗提物、总生物碱和中性部位的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。并将结果与参考抗生素进行比较。总生物碱对金黄色葡萄球菌有显著的抑菌活性(MIC=95mU g/ml)。(C)1999爱思唯尔爱尔兰科学有限公司。保留所有权利。
Holarrhena pubescens (syn. H. antidysenterica) (L.) WALL. stem bark was tested for antibacterial efficacy against Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus faecalis, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa using the microdilution broth method as well as the disc diffusion method. The crude methanolic extract was active against all tested bacteria. Further chemical fractionation indicated that the antibacterial activity was mainly associated with the alkaloids. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined for the crude extract, the total alkaloids and the neutral fraction using microdilution broth method. The results were compared with reference antibiotics. The total alkaloids showed remarkable activity against S. aureus (MIC = 95 mu g/ml). (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.