Genetic diversity among wild common beans from northwestern argentina based on morpho-agronomic and RAPD data

Genetic diversity among wild common beans from northwestern argentina based on morpho-agronomic and RAPD data
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DOI:
10.1007/s10722-004-0981-2
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发表时间:
2006-08-01
影响因子:
2
通讯作者:
Balatti, P. A.
Balatti, P. A.
中科院分区:
农林科学3区
文献类型:
--
作者:
Galvan, M. Z.;Menendez-Sevillano, M. C.;Balatti, P. A.

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利用RAPD标记和形态农艺资料分析了10个普通菜豆(Phaseolus vulgaris var. origineus)野生居群的遗传多样性。这项研究是从阿根廷西北部胡胡伊省、萨尔塔省和图库曼省不同地点收集的种群进行的。对10个数量性状和33个随机引物进行评分。基于形态农艺性状和RAPD标记的聚类产生了相似的表型图,根据收集地点对豆类群体进行分组。在人群中观察到的多样性水平很低,这表明他们有一个共同的祖先。形态农艺性状显示的多样性水平高于分子标记,这很可能是受环境的影响。此外,一个480 bp的DNA条带鉴定了从类似地点收集的一组野生种群。育种策略需要利用这种多样性来扩大商品豆的遗传基础,以开发高产品种。
The genetic diversity among 10 wild populations of common bean Phaseolus vulgaris var. aborigineus was analyzed by means of RAPD markers and morpho-agronomic data. The study was performed on populations collected from different sites located in the provinces of Jujuy, Salta and Tucuman in northwestern Argentina. Ten quantitative traits and 33 random primers were scored. Clustering based on morpho-agronomic traits and RAPD markers generated similar phenograms that grouped bean populations based on their site of collection. The levels of diversity observed among populations were low suggesting they have a common ancestor. The levels of diversity shown by morpho-agronomic traits were higher compared to those of molecular markers, most probably due to the effect of the environment. Furthermore, a 480-bp DNA band identified a group of wild populations collected from similar sites. Breeding strategies need to exploit this diversity to broaden the genetic base of commercial beans to develop high yield cultivars.