Multiplex Manager 1.0: a cross-platform computer program that plans and optimizes multiplex PCR
Multiplex Manager 1.0: a cross-platform computer program that plans and optimizes multiplex PCR
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DOI：
10.2144/000113156
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发表时间：
2009-06-01
期刊：
影响因子：
2.7
通讯作者：
Geerts, Paul G.
中科院分区：
文献类型：
--
作者：
Holleley, Clare E.;Geerts, Paul G.
Multiplex Manager 1.0是一个用户友好的跨平台程序，可以设计现有遗传标记位点的有效组合，形成多重聚合酶链反应，并利用先前的标记信息进行优化。该程序具有灵活性，可以解决两个设计问题：将所有标记组合成最小数量的反应，或者从许多可用标记中选择一个子集来设计高效且稳健的多路复用。我们的程序最大限度地减少了反应的数量、遗传联系和退火温度的差异。同时使标记间距、杂合性和等位基因数量最大化。最终输出提供了易于解释和信息丰富的反应图形表示，以及手动编辑最终反应的选项。Multiplex Manager 1.0可在www.multiplexmanager.com免费获得。
Multiplex Manager 1.0 is a user-friendly cross-platform program that designs efficient combinations of existing genetic marker loci into multiplex polymerase chain reactions and optimizes using prior marker information. The program has the flexibility to solve two design problems: combining all markers into the smallest number of reactions, or alternatively, selecting a subset from many available markers to design an efficient and robust multiplex. Our program minimizes the number of reactions, the genetic linkage, and the difference in annealing temperature. At the same time it maximizes the spacing between markers, the heterozygosity, and the number of alleles. The final output provides easily interpreted and informative graphical representations of reactions, as well as the option of manually editing final reactions. Multiplex Manager 1.0 is freely available at www.multiplexmanager.com.