Uptake and Depuration Kinetics of Pacific Ciguatoxins in Orange-Spotted Grouper (Epinephelus coioides)
Uptake and Depuration Kinetics of Pacific Ciguatoxins in Orange-Spotted Grouper (Epinephelus coioides)
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DOI：
10.1021/acs.est.9b07888
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发表时间：
2020-04-07
影响因子：
11.4
通讯作者：
Lam, Paul K. S.
中科院分区：
文献类型：
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作者：
Li, Jing;Mak, Yim Ling;Lam, Paul K. S.
雪卡毒素（CTXs）由有毒底栖鞭毛藻产生，可在较高营养水平的海洋生物中积累。目前的研究评估了一些最有效的ctx，太平洋CTX-1， -2和-3 （P-CTX-1， -2和-3）的吸收和净化动力学，橙斑石斑鱼（Epinephelus coioides）每天暴露于1 ng P-CTXs g（-1）鱼。在30 d的暴露过程中，P-CTX-1、-2和-3在暴露鱼的各种组织中一致检测到，组织中总p - ctx的浓度一般依次为肝、肠、鳃、皮肤、脑和肌肉。与P-CTX-2和-3相比，石斑鱼对P-CTX-1的吸收率相对较高。P- CTX-1、-2和-3在不同组织中的净化速率常数分别为（0.996-16.5）X 10（-2）、（1.51 16.1）X 10（-2）和（0.557-10.6）X 10(-2) d（-1）。P-CTX-1、-2和-3在全石斑鱼体内的积累效率分别为6.13%、2.61%和1.15%。在暴露阶段，P-CTX-1的比例增加，P-CTX-2和-3的比例下降，表明P-CTX-2和-3可能发生向P-CTX-1的生物转化，从而导致鱼体内P-CTX-1水平升高，长期暴露的鱼体内ctx的风险可能更高。
Ciguatoxins (CTXs), produced by toxic benthic dinoflagellates, can bioaccumulate in marine organisms at higher trophic levels. The current study evaluated the uptake and depuration kinetics of some of the most potent CTXs, Pacific CTX-1, -2, and -3 (P-CTX-1, -2, and -3), in orange-spotted grouper (Epinephelus coioides) exposed to 1 ng P-CTXs g(-1) fish daily. Over a 30 d exposure, P-CTX-1, -2, and -3 were consistently detected in various tissues of exposed fish, and the concentrations of the total P-CTXs in tissues generally ranked following the order of liver, intestine, gill, skin, brain, and muscle. Relatively higher uptake rates of P-CTX-1 in the groupers were observed compared with those of P-CTX-2 and -3. The depuration rate constants of P- CTX-1, -2, and -3 in different tissues were (0.996-16.5) X 10(-2), (1.51 16.1) X 10(-2), and (0.557-10.6) X 10(-2) d(-1), respectively. The accumulation efficiencies of P-CTX-1, -2, and -3 in whole groupers were 6.13%, 2.61%, and 1.15%, respectively. The increasing proportion of P-CTX-1 and the decreasing proportion of P-CTX-2 and -3 over the exposure phase suggest a likely biotransformation of P-CTX-2 and -3 to P-CTX-1, leading to higher levels of P-CTX-1 in fish and possibly a higher risk of CTXs in long-term exposed fish.