Vascular endothelial growth factor but not placental growth factor promotes trophoblast syncytialization in vitro
Vascular endothelial growth factor but not placental growth factor promotes trophoblast syncytialization in vitro
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DOI：
10.1016/s1071-5576(01)00134-4
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发表时间：
2001-11-01
期刊：
影响因子：
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通讯作者：
Baker, PN
中科院分区：
文献类型：
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作者：
Crocker, IP;Strachan, BK;Baker, PN
目的：本研究的目的是确定上皮生长因子（EGF）、血管内皮生长因子（VEGF）和胎盘生长因子（PIGF）对早期妊娠和足月妊娠细胞滋养层细胞分化的影响。从抽吸结束时获得的绒毛组织中分离妊娠早期滋养层细胞（n = 5），在选择性剖腹产时从胎盘（n = 6）分离足月滋养层细胞。在存在或不存在N-G-硝基-L-精氨酸甲酯盐酸盐（10（-4）M）的情况下，用0.5、5和50 ng/mL的EGF、V-EGF或PIGF刺激培养的细胞。通过抗桥粒蛋白和抗细胞角蛋白-7免疫染色鉴定合胞滋养层细胞，而细胞核计数在每个合胞体使用hematoxylin.RESULTS：没有治疗，背景水平的合胞显着在她的长期制剂比孕早期细胞。在VEGF和EGF的作用下，早期妊娠细胞滋养层细胞的合胞体数量和大小显著增加（P <0.05）。VEGF和EGF对培养细胞的合体化无明显影响。一氧化氮没有表现出参与合胞体诱导，和PIGF对细胞滋养层细胞的合胞化没有影响，无论是从第一或第三pregnesty.CONCLUSION：EGF和VEGF出现，以提高在体外合胞化的第一个三月的细胞滋养层细胞。（J Soc Gynecol Investig 2001; 8：341 - 6）版权所有（C）2001，由妇科研究学会所有。
OBJECTIVE: The object of this study tvas to determine the effect of epithelial growth factor (EGF), vascular endothelial growth factor (VEGF), and placental growth factor (PIGF) on the differentiation of first-trimester and term cytotrophoblasts.METHODS: The first-trimester trophoblasts were isolated from villous tissue obtained at suction termination (n = 5), and the term trophoblasts were isolated from placentas (n = 6) at elective cesarean. Cultured cells were stimulated with EGF, V-EGF, or PIGF at 0.5, 5, and 50 ng/mL, in the presence or absence of N-G-nitro-L-arginine methyl ester hydrochloride (10(-4) M). Syncytialized trophoblasts were identified by immunostaining with antidesmosomal protein and anti-cytokeratin-7, whereas nuclei were counted in each syncytia using hematoxylin.RESULTS: Without treatment, background levels of syncytialization were significantly ln her in term preparations than first-trimester cells. With VEGF and EGF, the number and size of syncytia increased significantly for the first-trimester cytotrophoblasts (P < .05). Neither VEGF nor EGF had any effect oil the syncytialization of cultured cells at term. Nitric oxide showed no involvement in syncytial induction, and PIGF had no effect on syncytialization of cytotrophoblasts, from either the first or third trimester.CONCLUSION: Both EGF and VEGF appeared to enhance the in vitro syncytialization of first trimester cytotrophoblasts. (J Soc Gynecol Investig 2001; 8:341-6) Copyright (C) 2001 by the Society for Gynecologic Investigation.