Stability of the conservative mode of nucleosome assembly.
Stability of the conservative mode of nucleosome assembly.
复制标题
核小体组装保守模式的稳定性。
DOI：
10.1093/nar/11.9.2717
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发表时间：
1983
影响因子：
14.9
通讯作者：
I. Leffak
中科院分区：
文献类型：
--
作者：
I. Leffak
平衡浮力密度离心后密度标记组蛋白的电泳分析证实了核小体组蛋白八聚体核心的保守组装。正常复制后，交联八聚体显示不包含新旧核心组蛋白的混合物。此外，当DNA合成被ara-C抑制时，核小体核心仍然完全由新生组蛋白组装。同样，在环己亚胺抑制释放后，新合成的核心组蛋白保守沉积。因此，当新生组蛋白在新生DNA的正常化学计量中存在时，当染色质在新生组蛋白或新生DNA过量中组装时，组蛋白八聚体组装的保守机制就会发生。
The conservative assembly of nucleosome histone octamer cores has been confirmed by electrophoretic analysis of density labeled histones following equilibrium buoyant density centrifugation. After normal replication, crosslinked octamers are shown not to contain a mixture of new and old core histones. Moreover, when DNA synthesis is inhibited by ara-C nucleosome cores are still assembled exclusively from nascent histone. Similarly, after release from cycloheximide inhibition newly synthesized core histone is conservatively deposited. Thus, a conservative mechanism of histone octamer assembly occurs when nascent histone is present in the normal stoichiometry to nascent DNA and when chromatin is assembled in nascent histone or nascent DNA excess.