LOCALIZED COLLAPSING CUES CAN STEER GROWTH CONES WITHOUT INDUCING THEIR FULL COLLAPSE
LOCALIZED COLLAPSING CUES CAN STEER GROWTH CONES WITHOUT INDUCING THEIR FULL COLLAPSE
复制标题
DOI：
10.1016/0896-6273(95)90284-8
复制
发表时间：
1995-02-01
期刊：
影响因子：
16.2
通讯作者：
RAPER, JA
中科院分区：
文献类型：
--
作者：
FAN, JH;RAPER, JA
塌陷因子是当以可溶性形式添加到培养的胚胎神经元中时诱导生长锥塌陷和麻痹的蛋白质。在这里，我们研究生长锥的本地化崩溃信号的反应。颞视网膜神经节细胞生长锥暴露于鼻视网膜神经节细胞轴突的局部塌陷刺激，经常顺利地远离轴突而不塌陷。转动是罕见的接触视网膜轴突不能诱导崩溃。在另一系列的实验中，背根神经节生长锥倾向于避开涂有富含运动抑制蛋白--黑素的脑提取物的珠子。许多旋转都是通过单独的丝状伪足接触来完成的。生长锥不转离涂有热灭活的胰蛋白酶的对照珠。这些结果表明，抑制性的指导线索可以引导生长锥通过局部抑制板状伪足突起。
Collapsing factors are proteins that induce growth cone collapse and paralysis when added in a soluble form to cultured embryonic neurons. Here we examine the responses of growth cones to localized collapsing signals. Temporal retinal ganglion cell growth cones exposed to a localized collapsing stimulus from nasal retinal ganglion cell axons frequently turn smoothly away from the axons without collapsing. Turning is rare on contact with retinal axons that are unable to induce collapse. In a separate series of experiments, dorsal root ganglion growth cones tend to turn away from beads coated with a brain extract enriched for the motility-inhibiting protein collapsin. Many turns are accomplished with filopodial contact alone. Growth cones do not turn away from control beads coated with heat-inactivated collapsin. These results suggest that inhibitory guidance cues can steer growth cones through a localized inhibition of lamellipodial protrusion.