Factors Influencing Embryogenesis in Carrot Cultures (Daucus carota L.)
Factors Influencing Embryogenesis in Carrot Cultures (Daucus carota L.)
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胡萝卜培养物中影响胚胎发生的因素（Daucus carota L.）
DOI：
10.1093/oxfordjournals.aob.a084898
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
L. H. Jones
中科院分区：
文献类型：
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作者：
L. H. Jones
毫无疑问，分离的空泡化胡萝卜细胞可以去分化并产生胚状体产生的胚性簇。然而，对培养物中胚性细胞起源的研究表明，最常见的来源是从原外植体中解放出来的小分生组织细胞群，并通过多次继代培养保持为分生组织细胞。空泡化细胞群分裂产生愈伤组织结节，其可以以多种方式进行形态发生。从一种细胞类型到另一种细胞类型的转变相对较少，细胞通常分裂产生与亲本相似类型的细胞。当培养基条件改变以有利于胚状体的增殖和发育时，接种物中低比例的胚性细胞的出现足以导致大量的胚状体。各种途径，植物可以从胡萝卜文化进行了讨论。
There is no doubt that isolated vacuolated carrot cells can de-differentiate and give rise to embryogenic clusters from which embryoids arise. However a study of the origins of embryogenic cells in culture suggests that the most frequent source is from groups of small meristematic cells liberated from the primary explant, and maintained as meristematic cells through numerous subcultures. Groups of vacuolated cells divide to give rise to callus nodules, which can undergo morphogenesis in a variety of ways. Transitions from one cell type to another are relatively infrequent, and cells generally divide to give rise to cells of similar type to the parent. The occurrence of a low proportion of embryogenic cells in an inoculum is sufficient to result in large numbers of embryoids when medium conditions are changed to favour their proliferation and development. The various routes by which plants can arise from carrot cultures are discussed.