Genetic control of the immune response: in vitro stimulation of lymphocytes by (T,G)-A--L, (H,G)-A--L, and (Phe,G)-A--L.
Genetic control of the immune response: in vitro stimulation of lymphocytes by (T,G)-A--L, (H,G)-A--L, and (Phe,G)-A--L.
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DOI：
10.1084/jem.140.4.977
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发表时间：
1974-10-01
期刊：
影响因子：
--
通讯作者：
McDevitt HO
中科院分区：
文献类型：
--
作者：
Lonai P;McDevitt HO
用体外抗原诱导的氚摄取方法研究了致敏淋巴细胞对(T，G)-A-L、(H，G)-A-L和(Phe，G)-A-L的反应。该反应依赖于T细胞和巨噬细胞。高纯度T细胞(91%Thy 1.2阳性)也有反应，这表明这个体外淋巴细胞转化系统不依赖于B细胞。体外刺激的高应答和低应答小鼠的淋巴细胞以应答和无应答的方式反应，其模式与体内免疫相同。只有在生理温度下加入可溶性抗原才能产生刺激；在4°C暴露抗原，然后在37°C洗涤和孵育，都不能诱导淋巴细胞转化。刺激对免疫抗原是特异的，不表现出这三种抗原及其各自的抗血清所特有的血清学交叉反应。该反应可被抗H-2血清抑制，但不能被抗免疫球蛋白血清抑制。然而，抗免疫球蛋白血清确实抑制了脂多糖或商陆有丝分裂原的刺激。这些结果表明，IR-1A基因(S)在T细胞中表达，T细胞和B细胞的抗原识别存在着根本的生理差异。
In vitro antigen-induced tritiated thymidine uptake has been used to study the response of sensitized lymphocytes to (T,G)-A--L, (H,G)-A--L, and (Phe,G)-A--L in responder and nonresponder strains of mice. The reaction is T-cell and macrophage dependent. Highly purified T cells (91% Thy 1.2 positive) are also responsive, suggesting that this in vitro lymphocyte transformation system is not B-cell dependent. Lymphocytes from high and low responder mice stimulated in vitro react as responders and nonresponders in a pattern identical to that seen with in vivo immunization. Stimulation occurs only if soluble antigen is added at physiological temperatures; antigen exposure at 4°C followed by washing and incubation at 37°C fails to induce lymphocyte transformation. Stimulation is specific for the immunizing antigen and does not exhibit the serologic cross-reactivity which is characteristic of these three antigens and their respective antisera. The reaction can be inhibited by anti-H-2 sera but not by anti-immunoglobulin sera. The anti-immunoglobulin sera did, however, inhibit lipopolysaccharide or pokeweed mitogen stimulation. These results suggest that the Ir-1A gene(s) are expressed in T cells, and that there are fundamental physiologic differences between T- and B-cell antigen recognition.