Effects of the protein corona on liposome-liposome and liposome-cell interactions.
Effects of the protein corona on liposome-liposome and liposome-cell interactions.
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DOI：
10.2147/ijn.s109059
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发表时间：
2016
影响因子：
8
通讯作者：
Tasciotti E
中科院分区：
文献类型：
--
作者：
Corbo C;Molinaro R;Taraballi F;Toledano Furman NE;Sherman MB;Parodi A;Salvatore F;Tasciotti E
彻底了解纳米载体与生物系统之间的相互作用对于预测和解释它们的生物分布、靶向性和有效性，从而设计更有效的药物输送系统至关重要。静脉注射后，纳米颗粒被蛋白质冠层(PC)包裹。这赋予了在很大程度上决定其生物命运的颗粒上的新的生物身份。脂质体具有强大的药物通用性，因此，作为概念的证明，它们的PC最近被认为与其内化到细胞内的机制和效率有关。为了更好地了解纳米载体与生物系统之间的相互作用，我们分析了吸附在多组分脂质体表面的血浆蛋白。具体地说，我们分析了脂质体/PC复合体的物理性质和超微结构，以及脂质体分散在血浆中时发生的聚集过程。共聚焦显微镜和流式细胞仪联合实验的结果表明，PC有利于脂质体被巨噬细胞和肿瘤细胞内化。这项工作深入了解了PC对脂质体物理性质的影响，从而影响了脂质体-脂质体和脂质体-细胞的相互作用。
A thorough understanding of interactions occurring at the interface between nanocarriers and biological systems is crucial to predict and interpret their biodistribution, targeting, and efficacy, and thus design more effective drug delivery systems. Upon intravenous injection, nanoparticles are coated by a protein corona (PC). This confers a new biological identity on the particles that largely determines their biological fate. Liposomes have great pharmaceutical versatility, so, as proof of concept, their PC has recently been implicated in the mechanism and efficiency of their internalization into the cell. In an attempt to better understand the interactions between nanocarriers and biological systems, we analyzed the plasma proteins adsorbed on the surface of multicomponent liposomes. Specifically, we analyzed the physical properties and ultrastructure of liposome/PC complexes and the aggregation process that occurs when liposomes are dispersed in plasma. The results of combined confocal microscopy and flow cytometry experiments demonstrated that the PC favors liposome internalization by both macrophages and tumor cells. This work provides insights into the effects of the PC on liposomes’ physical properties and, consequently, liposome–liposome and liposome–cell interactions.