Functional patterned multiphoton excitation deep inside scattering tissue
Functional patterned multiphoton excitation deep inside scattering tissue
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DOI：
10.1038/nphoton.2013.9
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发表时间：
2013-04-01
期刊：
影响因子：
35
通讯作者：
Emiliani, Valentina
中科院分区：
文献类型：
--
作者：
Papagiakoumou, Eirini;Begue, Aurelien;Emiliani, Valentina
散射样品中光束的随机失真使得脑组织中的深度光激发成为一个主要挑战。克服散射的常见解决方案涉及未知失真的自适应预补偿（1-3）。然而，这需要对样本特定的优化校正进行长时间的迭代搜索，这在应用于系统中的典型时间尺度在毫秒范围内的光学神经刺激时是一个问题。因此，在散射介质中的光激发是一个理想的解决方案，是一个特定的样品的属性无关。在这里，我们表明，具有广义相位对比（5）的时间聚焦双光子激发（4）使混浊组织内的任意空间图案的光激发具有显着的鲁棒性散射。我们证明了三维限制定制的光激发模式>200 μ m的深度，无论是在数值模拟和通过脑切片结合膜片钳记录的光激活通道视紫红质-2。
Stochastic distortion of light beams in scattering samples makes in-depth photoexcitation in brain tissue a major challenge. A common solution for overcoming scattering involves adaptive pre-compensation of the unknown distortion(1-3). However, this requires long iterative searches for sample-specific optimized corrections, which is a problem when applied to optical neurostimulation where typical timescales in the system are in the millisecond range. Thus, photoexcitation in scattering media that is independent of the properties of a specific sample would be an ideal solution. Here, we show that temporally focused two-photon excitation(4) with generalized phase contrast(5) enables photoexcitation of arbitrary spatial patterns within turbid tissues with remarkable robustness to scattering. We demonstrate three-dimensional confinement of tailored photoexcitation patterns >200 mu m in depth, both in numerical simulations and through brain slices combined with patch-clamp recording of photoactivated channelrhodopsin-2.