Lymphoid and myeloid differentiation of fetal liver CD34+lineage- cells in human thymic organ culture
Lymphoid and myeloid differentiation of fetal liver CD34+lineage- cells in human thymic organ culture
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人胸腺器官培养物中胎肝 CD34 谱系细胞的淋巴和骨髓分化
DOI：
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发表时间：
1994
影响因子：
15.3
通讯作者：
H. Spits
中科院分区：
文献类型：
--
作者：
A. Bárcena;A. Galy;J. Punnonen;M. Muench;D. Schols;M. Roncarolo;J. D. de Vries;H. Spits
在本文中，我们报告，人胎儿胸腺含有CD 34 bright细胞（< 0.01%的总胸腺细胞），其表型类似于胎儿骨髓中的多能造血祖细胞。CD 34亮型胸腺细胞为CD 33-/暗型，CD 38、CD 2和CD 5以及谱系标志物CD 3、CD 4和CD 8（T细胞）、CD 19和CD 20（B细胞）、CD 56（NK细胞）、血型糖蛋白（红细胞）和CD 14（单核细胞）均为阴性。此外，总CD 34+谱系阴性（lin-）胸腺细胞含有少量的原始髓系祖细胞，因此表明胸腺中存在的不同造血谱系可能来源于接种胸腺的原始造血祖细胞。为了研究胸腺是否允许非T细胞的发育，通过将分选的CD 34 +lin-胎肝细胞显微注射到HLA-不匹配的胎胸腺片段中来进行人胎器官培养（FTOC）测定。对FTOC衍生的CD 34 +lin-细胞后代的连续表型分析表明，在分化为T细胞之前，有一波髓样分化为CD 14 + CD 11b + CD 4dull细胞。还产生了供体来源的B细胞（CD 19 + CD 20+），当在适当条件下培养时，其产生免疫球蛋白（IgG和IgM），以及功能性CD 56 + CD 3- NK细胞，其在细胞毒性试验中有效杀死K562靶细胞。这些结果表明，显微注射胎肝造血祖细胞到胎儿胸腺器官片段的结果在体外多谱系分化。
In this article, we report that the human fetal thymus contains CD34bright cells (< 0.01% of total thymocytes) with a phenotype that resembles that of multipotent hematopoietic progenitors in the fetal bone marrow. CD34bright thymocytes were CD33-/dull and were negative for CD38, CD2, and CD5 as well as for the lineage markers CD3, CD4, and CD8 (T cells), CD19 and CD20 (B cells), CD56 (NK cells), glycophorin (erythrocytes), and CD14 (monocytes). In addition, total CD34+ lineage negative (lin-) thymocytes contained a low number of primitive myeloid progenitor cells, thus suggesting that the different hematopoietic lineages present in the thymus may be derived from primitive hematopoietic progenitor cells seeding the thymus. To investigate whether the thymus is permissive for the development of non-T cells, human fetal organ culture (FTOC) assays were performed by microinjecting sorted CD34+lin- fetal liver cells into fragments of HLA- mismatched fetal thymus. Sequential phenotypic analysis of the FTOC- derived progeny of CD34+lin- cells indicated that the differentiation into T cells was preceded by a wave of myeloid differentiation into CD14+CD11b+CD4dull cells. Donor-derived B cells (CD19+CD20+) were also generated, which produced immunoglobulins (IgG and IgM) when cultured under appropriate conditions, as well as functional CD56+CD3- NK cells, which efficiently killed K562 target cells in cytotoxicity assays. These results demonstrate that the microinjection of fetal liver hematopoietic progenitors into fetal thymic organ fragments results in multilineage differentiation in vitro.
前列腺素 E 和重组干扰素 α、β 和 γ 对人骨髓粒细胞巨噬细胞祖细胞的协同抑制以及肿瘤坏死因子介导的作用。
DOI：
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发表时间：
1988
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Pelus,LM;Ottmann,OG;Nocka,KH
通讯作者：
Nocka,KH
未成熟的人类胸腺细胞可以被胸腺上皮细胞的细胞因子驱动分化为非淋巴谱系。
DOI：
10.1073/pnas.86.19.7575
发表时间：
1989
影响因子：
11.1
作者：
Kurtzberg,J;Denning,SM;Nycum,LM;Singer,KH;Haynes,BF
通讯作者：
Haynes,BF