A Strain of Pseudomonas sp. Isolated from Piggery Wastewater Treatment Systems with Heterotrophic Nitrification Capability in Taiwan
A Strain of Pseudomonas sp. Isolated from Piggery Wastewater Treatment Systems with Heterotrophic Nitrification Capability in Taiwan
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DOI：
10.1007/s00284-006-0021-x
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发表时间：
2006-06
影响因子：
2.6
通讯作者：
Jung-Jeng Su;K. Yeh;P. Tseng
中科院分区：
文献类型：
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作者：
Jung-Jeng Su;K. Yeh;P. Tseng
养猪场废水中高浓度的NH4+是台湾的主要问题。因此，在我们的研究中，我们分离了原生异养硝化菌用于养猪场废水的处理。从养猪场污水处理系统的活性污泥中分离出异养硝化菌AS-1，并对其进行了表征。采用三件套卷曲密封血清瓶，在30°C培养箱中验证菌株AS-1的异养硝化能力。所有血清瓶含有80 mL培养基，瓶顶空间的剩余部分充满纯氧。实验结果表明，58 h脱除2.5±0.2 mmol L - 1NH4+，最终得到1.5±0.5 mmol L - 1n2和0.2±0.0 mmol L - 1N2O。菌株AS-1对NH4+的去除率为1.75 mmol NH4+g cell−1h−1。对该菌株进行16S rDNA测序，并与其他微生物进行比较，鉴定为产酸假单胞菌（asPseudomonas alcaligenes），鉴定率为97%。因此，菌株AS-1在猪舍废水中原位去除NH4+方面具有很高的应用前景。
A high concentration of NH4+in piggery wastewater is major problem in Taiwan. Therefore, in our study, we isolated native heterotrophic nitrifiers for piggery wastewater treatment. Heterotrophic nitrifier AS-1 was isolated and characterized from the activated sludge of a piggery wastewater system. Sets of triplicate crimp-sealed serum bottles were used to demonstrate the heterotrophic nitrifying capability of strain AS-1 in an incubator at 30°C. All serum bottles contained 80 mL medium, and the remainder of the bottle headspace was filled with pure oxygen. The experimental results showed that 2.5 ± 0.2 mmol L−1NH4+was removed by 58 hours, and, eventually, 1.5 ± 0.5 mmol L−1N2and 0.2 ± 0.0 mmol L−1N2O were produced. The removal rate of NH4+by the strain AS-1 was 1.75 mmol NH4+g cell−1h−1. This strain was then identified asPseudomonas alcaligenes(97% identity) by sequencing its 16S rDNA and comparing it with other microorganisms. Thus, strain AS-1 displays high promise for future application for in situ NH4+removal from piggery wastewater.