Different myrosinase and idioblast distribution in Arabidopsis and Brassica napus
Different myrosinase and idioblast distribution in Arabidopsis and Brassica napus
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DOI：
10.1104/pp.010334
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发表时间：
2001-12-01
期刊：
影响因子：
7.4
通讯作者：
Meijer, J
中科院分区：
文献类型：
--
作者：
Andréasson, E;Jorgensen, LB;Meijer, J
黑芥子酶 (EC 3.2.3.1) 是一种芥子油苷降解酶，主要存在于十字花科植物的特殊成芽细胞、黑芥子细胞中。这种由次级产物和降解酶组成的双组分系统在植物与昆虫的相互作用中非常重要。拟南芥的免疫细胞化学分析将黑芥子酶仅定位于韧皮部薄壁组织中的黑芥子细胞，而在地面组织中未检测到黑芥子细胞。在甘蓝型油菜中，韧皮部薄壁组织和地面组织中的黑芥子细胞中都可以检测到黑芥子酶。拟南芥和欧洲油菜中的黑芥子细胞是相似的，并且被发现与拟南芥中存在的伴随细胞和含芥子油苷的S细胞不同。对甘蓝型油菜胚胎中芥子细胞的共聚焦激光扫描免疫显微镜分析表明，芥子蛋白颗粒在细胞中构成了连续的网状系统。这些发现表明，在所研究的两个物种中，产生基础组织的初始细胞具有不同的产生成成细胞的潜力，并表明黑芥子酶-芥子油苷系统至少具有部分不同的功能。欧洲油菜提取物中的几种黑芥子酶与黑芥子酶结合蛋白 (MBP) 一起以复合物的形式回收，因此对 MBP 的定位进行了原位研究。 MBP 在甘蓝型油菜发芽幼苗中的表达量最高，并且在除地面组织的芥子细胞外的所有细胞中都存在。非黑芥子细胞中 MBP 的快速消失和黑芥子细胞中 MBP 的出现导致 7 日龄幼苗中 MBP 和黑芥子酶明显共定位。
Myrosinase (EC 3.2.3.1) is a glucosinolate-degrading enzyme mainly found in special idioblasts, myrosin cells, in Brassicaceae. This two-component system of secondary products and degradative enzymes is important in plant-insect interactions. Immunocytochemical analysis of Arabidopsis localized myrosinase exclusively to myrosin cells in the phloem parenchyma, whereas no myrosin cells were detected in the ground tissue. In Brassica napus, myrosinase could be detected in myrosin cells both in the phloem parenchyma and in the ground tissue. The myrosin cells were similar in Arabidopsis and B. napus and were found to be different from the companion cells and the glucosinolate-containing S-cells present in Arabidopsis. Confocal laser scanning immunomicroscopy analysis of myrosin cells in B. napus embryos showed that the myrosin grains constitute a continuous reticular system in the cell. These findings indicate that in the two species studied, initial cells creating the ground tissue have different potential for making idioblasts and suggest that the myrosinase-glucosinolate system has at least partly different functions. Several myrosinases in B. napus extracts are recovered in complex together with myrosinase-binding protein (MBP), and the localization of MBP was therefore studied in situ. The expression of MBP was highest in germinating seedlings of B. napus and was found in every cell except the myrosin cells of the ground tissue. Rapid disappearance of the MBP from the non-myrosin cells and emergence of MBP in the myrosin cells resulted in an apparent colocalization of MBP and myrosinase in 7-d-old seedlings.