Ellagic acid prevents gut damage via ameliorating microbe-associated intestinal lymphocyte imbalance.
Ellagic acid prevents gut damage via ameliorating microbe-associated intestinal lymphocyte imbalance.
复制标题
鞣花酸通过改善微生物相关的肠道淋巴细胞失衡来防止肠道损伤。
DOI：
10.1039/d2fo01512a
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发表时间：
2022
期刊：
影响因子：
6.1
通讯作者：
Miaomiao Wu
中科院分区：
文献类型：
--
作者：
Huimin Jin;Siyan Che;Kunfu Wu;Miaomiao Wu
炎症性肠病（IBD）的发病机制涉及肠道应激后持续的微生物介导的免疫应答。虽然抗生素的管理可以是一种有效的治疗，抗生素的滥用可能会导致未知的耐药细菌的风险。采用鞣花酸（EA）和氨苄青霉素（AMP）预处理仔猪21 d，分别于第14和18 d腹腔注射百草枯（PQ）。我们发现AMP组的仔猪失去了大部分肠道微生物，免受肠道氧化应激引起的后续肠道损伤。因此，我们确定了一些肠道微生物，它们可能在PQ诱导的应激中介导细胞因子刺激后的细胞反应中发挥关键作用。EA预处理表现出与AMP相同的性能。EA预处理降低了肠道中链球菌的丰度。电针可调节肠淋巴细胞的分布，降低肠上皮细胞中CD 79a+细胞的比例，抑制CD 3+细胞向肠绒毛顶端的迁移。此外，肠道免疫应答与肠道中链球菌的丰度密切相关。因此，我们得出结论，EA有可能取代抗生素，以防止肠道微生物介导的免疫反应，EA可以作为一种补充候选，以减轻肠道应激引起的炎症的发展。
Inflammatory bowel disease (IBD) pathogenesis involves a sustained microbial-mediated immune response following intestinal stress. Although administration of antibiotics can be an effective therapy, the misuse of antibiotics may risk unknown drug-resistant bacteria. In this study, piglets pretreated with ellagic acid (EA) and Ampicillin (AMP) for 21 days, and were injected intraperitoneally with paraquat (PQ) on 14 and 18 days. We found piglets lost most of their gut microbes in the AMP group, protected from subsequent intestinal damage caused by gut oxidative stress. Hence, we identified some gut microbes that may play a critical role in mediating cellular responses following cytokine stimulation in PQ-induced stress. EA preprocessing exhibited the same performance as AMP. Pretreatment of EA reduced Streptococcus abundance in the gut. Particularly, EA modulated intestinal lymphocyte distribution, reduced the frequency of CD79a+ cells, and alleviated the upward migration of CD3+ cells to the apex of the intestinal villi in the intestinal epithelium. Additionally, the intestinal immune response had been known associated closely with the abundance of Streptococcus in the gut. Thus, we concluded that EA has the potential to replace antibiotics to prevent microbial-mediated immune responses in the gut, and EA can be applied as a supplement candidate to alleviate the development of inflammation caused by intestinal stress.