Analysis of dissimilatory sulfite reductase and 16S rRNA gene fragments from deep-sea hydrothermal sites of the Suiyo Seamount, Izu-Bonin Arc, Western Pacific
Analysis of dissimilatory sulfite reductase and 16S rRNA gene fragments from deep-sea hydrothermal sites of the Suiyo Seamount, Izu-Bonin Arc, Western Pacific
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DOI：
10.1128/aem.70.1.393-403.2004
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发表时间：
2004-01-01
影响因子：
4.4
通讯作者：
Fukui, M
中科院分区：
文献类型：
--
作者：
Nakagawa, T;Ishibashi, JI;Fukui, M
这项研究描述了日本西太平洋伊豆-小笠原岛弧水阳海山深海热液喷口场 1,380 m 深处独特的异化亚硫酸盐还原酶 (DSR) 基因的出现情况。 DSR 基因是从在通风口部署的导管型原位生长室中生长 3 天的微生物以及 5 摄氏度的钻孔废水和 7 摄氏度的自然通风口流体中获得的。 DSR克隆SUIYOdsr-A和SUIYOdsr-B与栽培物种或环境克隆没有密切关系。此外，通过对 16S rRNA 基因进行 PCR 变性梯度凝胶电泳 (DGGE) 分析来检查微生物群落样本。培养 3 天后，对从通气导管获得的 16S rRNA 基因片段进行序列分析，揭示了与 Aquiificae 和 γ- 和 epsilon-Proteobacteria 相关的细菌 DGGE 带的出现，以及与 Thermococcales 相关的古菌系统型的出现以及与“纳米古菌群”聚集的独特古菌序列的出现。从 7 至 300 摄氏度的钻孔和天然喷口流体中获得的 DGGE 带属于 delta-Proteobacteria、Thiomicrospira 属和 Pelodictyon。从 3 和 4℃ 套管流出水中检索到的 DGGE 优势带与从北冰洋获得的系统发育型密切相关。我们的结果表明，存在与之前在其他地热环境中未检测到的独特 DSR 谱系相对应的微生物。
This study describes the occurrence of unique dissimilatory sulfite reductase (DSR) genes at a depth of 1,380 m from the deep-sea hydrothermal vent field at the Suiyo Seamount, Izu-Bonin Arc, Western Pacific, Japan. The DSR genes were obtained from microbes that grew in a catheter-type in situ growth chamber deployed for 3 days on a vent and from the effluent water of drilled holes at 5degreesC and natural vent fluids at 7degreesC. DSR clones SUIYOdsr-A and SUIYOdsr-B were not closely related to cultivated species or environmental clones. Moreover, samples of microbial communities were examined by PCR-denaturing gradient gel electrophoresis (DGGE) analysis of the 16S rRNA gene. The sequence analysis of 16S rRNA gene fragments obtained from the vent catheter after a 3-day incubation revealed the occurrence of bacterial DGGE bands affiliated with the Aquificae and gamma- and epsilon-Proteobacteria as well as the occurrence of archaeal phylotypes affiliated with the Thermococcales and of a unique archaeon sequence that clustered with "Nanoarchaeota." The DGGE bands obtained from drilled holes and natural vent fluids from 7 to 300degreesC were affiliated with the delta-Proteobacteria, genus Thiomicrospira, and Pelodictyon. The dominant DGGE bands retrieved from the effluent water of casing pipes at 3 and 4degreesC were closely related to phylotypes obtained from the Arctic Ocean. Our results suggest the presence of microorganisms corresponding to a unique DSR lineage not detected previously from other geothermal environments.