Characterization of heat injury in grapes using h nuclear magnetic resonance methods : changes in transverse relaxation times.
Characterization of heat injury in grapes using h nuclear magnetic resonance methods : changes in transverse relaxation times.
复制标题
使用核磁共振方法表征葡萄热损伤：横向弛豫时间的变化。
DOI：
10.1104/pp.96.3.957
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发表时间：
1991
期刊：
影响因子：
7.4
通讯作者：
C. Rajashekar
中科院分区：
文献类型：
--
作者：
M. Abass;C. Rajashekar
研究了葡萄杂交种（葡萄属）叶片和悬浮培养细胞组织水（（1）H）的横向弛豫时间（T（2））。cv ;Venus'和Veeblanc'）在不同温度下通过核磁共振测量。组织水的特征在于两个T（2）时间常数。通过测定叶片和悬浮培养细胞的电解质渗漏和氯化三苯基四氮唑还原，观察到与热损伤相关的组织水主要部分的T（2）急剧下降。热损伤引起的T（2）的变化是不可逆的，如T（2）的温度依赖性滞后所示。使用顺磁性探针（Mn（+2））的研究表明，质膜在杀灭温度下受到不可逆的损伤，导致细胞区室化丧失。热灭活样本中的组织水仅由单个T（2）表征。
Transverse relaxation times (T(2)) of tissue water ((1)H) in leaves and suspension cultured cells of grape hybrids (Vitis spp. cv ;Venus' and ;Veeblanc') were measured by nuclear magnetic resonance at various temperatures. The tissue water was characterized by two T(2) time constants. A sharp decrease in T(2) for the major fraction of tissue water was observed in association with heat injury, as measured by electrolyte leakage and triphenyltetrazolium chloride reduction in both leaves and suspension cultured cells. The changes in T(2) as a result of heat injury were irreversible, as indicated by a temperature dependent hysteresis of T(2). Studies using a paramagnetic probe (Mn(+2)) indicated that the plasma membrane was irreversibly damaged at the killing temperature, resulting in a loss of cell compartmentalization. Tissue water in heat-killed samples was characterized by only a single T(2).