Glucosinolates and other metabolites in the leaves of Arabidopsis thaliana from natural populations and their effects on a generalist and a specialist herbivore
Glucosinolates and other metabolites in the leaves of Arabidopsis thaliana from natural populations and their effects on a generalist and a specialist herbivore
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DOI：
10.1007/s00049-007-0394-8
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发表时间：
2008-06-01
期刊：
影响因子：
1.8
通讯作者：
van der Meijden, E.
中科院分区：
文献类型：
--
作者：
Arany, A. Mosleh;de Jong, T. J.;van der Meijden, E.
由于植物中的许多次生代谢物可以防御食草动物，因此推测这些化合物是在昆虫食草动物的选择压力下进化的。对特定物种代谢物模式的物种内差异的一种解释是，不同的种群受到不同食草动物的选择。我们使用源自沙丘和内陆地区的拟南芥植物检验了这一假设。我们使用核磁共振波谱和多变量数据分析分析了拟南芥叶子。化学成分的主要差异存在于水-甲醇组分中，这是由于沙丘植物中黑豆苷和富马酸的浓度较高。内陆植物的葡萄糖水平较低。使用 HPLC 进行芥子油苷的定量分析。个别植物和种群在硫代葡萄糖苷的组成和浓度方面表现出差异。在生长室实验中，多面食草动物甜菜夜蛾在内陆植物上生长得明显更好，而专食草食动物小菜蛾在内陆植物上的表现同样好。脂肪族芥子油苷以及总芥子油苷浓度与甜菜夜蛾幼虫质量呈负相关。然而，小菜蛾幼虫质量与叶片中硫代葡萄糖苷之间没有发现显着相关性。专家和通才食草动物对植物次级化学的反应不同，其他几项研究也发现了这一点。这是一个重要的迹象，表明种群之间芥子油苷浓度的差异可能是由于不同食草动物群体的差异选择造成的。
Because many secondary metabolites in plants act as defense against herbivores it has been postulated that these compounds have evolved under selective pressure by insect herbivores. One explanation for the within-species variation in metabolite patterns in a particular species is that different populations are under selection by different herbivores. We tested this hypothesis, using Arabidopsis thaliana plants that originated from dune and inland areas. We analyzed Arabidopsis thaliana leaves using NMR spectroscopy and multivariate data analysis. Major differences in chemical composition were found in water-methanol fractions and were due to higher concentrations of sinigrin and fumaric acid in dune plants. Inland plants showed lower levels of glucose. Quantitative analysis of glucosinolates was performed with HPLC. Individual plants and populations demonstrated differences in glucosinolate composition and concentration. In growth chamber experiments, the generalist herbivore, Spodoptera exigua grew significantly better on the inland plants, while the specialist herbivore Plutella xylostella performed equally well on plants of both origins. Aliphatic glucosinolate as well as total glucosinolate concentrations negatively correlated with larval mass of Spodoptera exigua. No significant correlations, however, were found between larval mass of Plutella xylostella and glucosinolates in the leaves. A specialist and a generalist herbivore were responding differently to plant secondary chemistry, as was also found in several other studies. This is an important indication that differences in glucosinolate concentrations among populations may result from differential selection by different guilds of herbivores.