Women with high telomerase activity in luteinised granulosa cells have a higher pregnancy rate during in vitro fertilisation treatment
Women with high telomerase activity in luteinised granulosa cells have a higher pregnancy rate during in vitro fertilisation treatment
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DOI：
10.1007/s10815-011-9600-2
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发表时间：
2011-09-01
影响因子：
3.1
通讯作者：
Yang, Dongzi
中科院分区：
文献类型：
--
作者：
Chen, Hong;Wang, Wenjun;Yang, Dongzi
目的探讨人黄体化颗粒细胞（GCs）端粒酶活性（TA）对体外受精效果的影响。方法将56名年龄在23 ~ 39岁的女性按TA水平分为4组。结果A组17例TA未检出，B组16例TA低表达（0.1 ~ 0.65 OD x mm）， C组14例TA中表达（0.66 ~ 1.00 OD x mm）， D组9例TA高表达（> 1.00 OD x mm）。C组和D组血清总睾酮(T)水平显著高于A组（1.43 +/- 0.10 vs. 1.08 +/- 0.11 nmol/L, P < 0.030; 1.56 +/- 0.08 vs. 1.08 +/- 0.11 nmol/L, P < 0.005）。TA水平与T呈正相关（r = 0.291, P < 0.011）。各组受精率、卵裂率、成熟卵母细胞形成率和胚胎质量均无明显差异。D组患者胚胎着床率和临床妊娠率最高，分别为52.63%和77.78%，A组为18.92%和29.41%，B组为25.71%和37.50%，C组为48%和50%，P < 0.018, P = 0.112。与a组（OR: 9.703, P < 0.023）、B组（OR: 14.765, P < 0.009）、C组（OR: 5.560, P = 0.103）相比，D组患者的妊娠可能性更大。结论黄体化GCs具有一定的增殖潜力，黄体化GCs TA可预测体外受精治疗的临床结局。一些未知的调控机制之间的TA和T应该在进一步的试验研究。
Objective To study the effect of telomerase activity (TA) in human luteinised granulosa cells (GCs) on the outcome of in vitro fertilisation treatment.Methods Fifty-six women, aged 23 to 39 years, were enrolled and divided into four groups according to their levels of TA.Results Seventeen cases in group A exhibited nondetectable TA, 16 cases in group B expressed low levels of TA (between 0.1 and 0.65 OD x mm), 14 cases in group C expressed moderate TA levels (between 0.66 and 1.00 OD x mm) and 9 cases in group D expressed high levels of TA (more than 1.00 OD x mm). The level of total serum testosterone (T) was significantly higher in groups C and D than in group A (1.43 +/- 0.10 vs. 1.08 +/- 0.11 nmol/L, P < 0.030 and 1.56 +/- 0.08 vs. 1.08 +/- 0.11 nmol/L, P < 0.005, respectively). The TA level was positively correlated with T (r = 0.291, P < 0.011). No obvious differences were observed in rates of fertilisation, cleavage, mature oocyte formation or good-quality embryos among the groups. The patients in group D exhibited the highest rates of embryo implantation and clinical pregnancy (with rates of 52.63% and 77.78%, respectively, compared with 18.92% and 29.41% in group A, 25.71% and 37.50% in group B and 48% and 50% in group C, with P < 0.018 and P = 0.112, respectively). The patients in group D also had a greater likelihood of becoming pregnant than those in group A (OR: 9.703, P < 0.023), group B (OR: 14.765, P < 0.009) or group C (OR: 5.560, P = 0.103).Conclusions Luteinised GCs have a certain potential for proliferation and TA of luteinised GCs may predict the clinical outcomes of IVF treatment. Some unknown regulatory mechanisms between TA and T should be studied in further trials.