Jiedu Huoxue decoction improves chronic abacterial prostatitis/chronic pelvic pain syndrome through activating Wnt/GSKβ/β-catenin signaling pathway and alleviating apoptosis.
Jiedu Huoxue decoction improves chronic abacterial prostatitis/chronic pelvic pain syndrome through activating Wnt/GSKβ/β-catenin signaling pathway and alleviating apoptosis.
复制标题
解毒活血汤通过激活Wnt/GSKβ/β-catenin信号通路、减轻细胞凋亡来改善慢性非细菌性前列腺炎/慢性盆腔疼痛综合征。
DOI：
10.1016/j.biopha.2022.112830
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Zhangren Yan
中科院分区：
文献类型：
--
作者：
Jun Yi;Junqing Pan;Sina Zhang;Wenli Mao;Jiangang Wang;Wanchun Wang;Zhangren Yan
慢性非细菌性前列腺炎/慢性盆腔疼痛综合征（CP/CPPS）占临床前列腺炎的90%，目前尚无特异有效的治疗方法，解毒活血汤被认为是治疗CP/CPPS的有效药物，但其具体作用机制尚不清楚。观察前列腺指数、尿量、管腔面积、上皮厚度和疼痛反应时间。TUNEL染色和流式细胞术被施加到测量细胞凋亡水平在体内和vitrial.ResultsJDHXD提高CP/CPPS恢复以剂量依赖的方式。JDHXD抑制CP/CPPS动物模型的细胞凋亡，激活Wnt/GSK-3β/β-catenin信号通路。抑制Wnt/GSK-3β/β-catenin信号通路可明显促进细胞凋亡，抑制JDHXD对CP/CPPS的改善作用。结论解毒活血汤通过激活Wnt/GSKβ/β-catenin信号通路，抑制细胞凋亡，改善CP/CPPS。本研究为通过激活Wnt/GSK-3β/β-catenin信号通路防治CP/CPPS提供了新的思路。
BackgroundChronic abacterial prostatitis/chronic pelvic pain syndrome (CP/CPPS) accounts for 90% of clinical prostatitis, and there is no specific and effective treatment for it. Jiedu Huoxue decoction (JDHXD) has been believed to be an effective agent for CP/CPPS, but the specific mechanism remains unclear.MethodsCarrageenan and LPS were used to established the animal and cell models of CP/CPPS, respectively. The prostate index, urine volume, lumen area, epithelial thickness, and pain response time were investigated. TUNEL staining and flow cytometry were applied to measure apoptosis levels in vivo and in vitro.ResultsJDHXD improved CP/CPPS recovery in a dose-dependent manner. JDHXD restrained apoptosis and activated Wnt/GSK-3β/β-catenin signaling pathway in the CP/CPPS animal model. Inhibition of Wnt/GSK-3β/β-catenin signaling pathway remarkably aggravated apoptosis and suppressed the improvement of CP/CPPS by JDHXD. XAX939 markedly reversed the suppression of cell apoptosis and ROS level caused by JDHXD in vitro.ConclusionJiedu Huoxue decoction improved CP/CPPS through activating Wnt/GSKβ/β-catenin signaling pathway and inhibiting apoptosis. This study might provide a novel insight for the prevention and treatment of CP/CPPS through activating Wnt/GSK-3β/β-catenin signaling pathway.