Leukotriene synthesis is required for hedgehog-dependent neurite projection in neuralized embryoid bodies but not for motor neuron differentiation
Leukotriene synthesis is required for hedgehog-dependent neurite projection in neuralized embryoid bodies but not for motor neuron differentiation
复制标题
DOI：
10.1634/stemcells.2007-0841
复制
发表时间：
2008-05-01
期刊：
影响因子：
5.2
通讯作者：
Roelink, Henk
中科院分区：
文献类型：
--
作者：
Bijlsma, Maarten F.;Peppelenbosch, Maikel P.;Roelink, Henk
hedgehog （Hh）通路是许多发育过程所必需的。以及成人体内平衡。虽然所有已知的Hh信号对模式和分化的影响都是由锌环铃转录因子Gli家族的成员介导的，但我们证明了Hh依赖于运动神经元形成的神经突，就像成纤维细胞的迁移一样，需要白三烯合成，这与Gli介导的Hh反应不同。白三烯代谢需要平滑的活性，相反，白三烯代谢需要介导Hh对神经突投射的影响。这些数据建立了先前描述的花生四烯酸依赖Hh通路在发育相关模型系统中的功能。
The hedgehog (Hh) pathway is required for many developmental processes,. as well as for adult homeostasis. Although all known effects of Hh signaling affecting patterning and differentiation are mediated by members of the Gli family of zinc ringer transcription factors, we demonstrate that the Hh-dependent formation of neurites from motor neurons, like migration of fibroblasts, requires leukotriene synthesis and is different from the Gli-mediated Hh response. Smoothened activity is required for the use of the leukotriene metabolism, and inversely, the leukotriene metabolism is required for mediating the Hh effects on neurite projection. These data establish a function for the previously described arachidonic acid-dependent Hh pathway in a developmentally relevant model system.