Macroscopic investigation of the transient hydrodynamic memory behavior of preparative packed chromatography beds.
Macroscopic investigation of the transient hydrodynamic memory behavior of preparative packed chromatography beds.
复制标题
制备型填充色谱床瞬态流体动力学记忆行为的宏观研究。
DOI：
10.1016/j.chroma.2010.12.092
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
D. Weuster‐Botz
中科院分区：
文献类型：
--
作者：
D. Hekmat;R. Mornhinweg;G. Bloch;Yu Sun;P. Jeanty;M. Neubert;D. Weuster‐Botz
本工作的目的是检查的流体动力学行为的制备规模的填充色谱床在长期的周期性操作在高负荷使用的实验装置与高分辨率的床高测量装置。在140 mm柱中检测了一种琼脂糖基树脂和一种甲基丙烯酸基树脂。两种树脂在压缩/松弛循环期间均表现出滞后行为。随着水动力应力率的降低，这些现象不那么明显。吸附树脂的出现表明了色谱填料的流体力学记忆行为。在甲基丙烯酸树脂填充柱的高负荷长期循环操作中，首次观察到流速随时间稳定下降的振荡。这些振荡归因于聚合物颗粒网络的粘弹性，其代表具有衰减记忆的材料的系统。已知这种具有反馈的非线性系统表现出固有的自振荡。观察到床层压缩和床层渗透率降低这两个过程的解耦。所呈现的结果解释了为什么制备填充床色谱法经常产生不令人满意的可重复数据，以及为什么经常观察到不必要的现象，如中壁分离或其他恶化的色谱床症状。
The goal of the present work was to examine the hydrodynamic behavior of preparative scale packed chromatography beds during long-term cyclical operation at high loads using an experimental set-up with a high resolution measuring device of bed height. One agarose-based resin and one methacrylic-based resin were examined in a 140mm column. Both resins exhibited hysteresis behavior during compression/relaxation cycles. The hystereses were less pronounced with decreasing hydrodynamic stress rate. The occurrence of hystereses was an indication for hydrodynamic memory behavior of the chromatography packing. During long-term cyclical operation at high loads of the column filled with methacrylic resin, oscillations of the steadily with time decreasing flow rate were observed for the first time. These oscillations were attributed to the viscoelasticity of the polymer particles network representing a system with materials with fading memory. Such nonlinear systems with feed-back are known to exhibit inherent self-oscillations. A decoupling of the two processes of bed compression and decrease of bed permeability was observed. The presented results explain why preparative packed-bed chromatography often yields unsatisfactory reproducible data and why unwanted phenomena like medium wall detachment or other symptoms of deteriorated chromatography beds are frequently observed.