PREPARATION OF A DNA MATRIX VIA AN ELECTROCHEMICALLY DIRECTED COPOLYMERIZATION OF PYRROLE AND OLIGONUCLEOTIDES BEARING A PYRROLE GROUP
PREPARATION OF A DNA MATRIX VIA AN ELECTROCHEMICALLY DIRECTED COPOLYMERIZATION OF PYRROLE AND OLIGONUCLEOTIDES BEARING A PYRROLE GROUP
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DOI：
10.1093/nar/22.15.2915
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发表时间：
1994-08-11
影响因子：
14.9
通讯作者：
TEOULE, R
中科院分区：
文献类型：
--
作者：
LIVACHE, T;ROGET, A;TEOULE, R
本文介绍了一种制备定向DNA基质的新方法。该方法包括吡咯和寡核苷酸的电化学定向共聚，所述寡核苷酸在其5'端上带有通过亚磷酰胺化学引入的吡咯部分。共聚物（聚吡咯）的电控合成在一个步骤中给出沉积在电极表面上的固体导电膜。所得聚合物由带有共价连接的寡核苷酸的吡咯链组成。聚合物生长限于电极表面，因此可以通过连续共聚在多电极装置上制备DNA基质。使用带有四个寡核苷酸的支持物检测合成DNA片段上的三个ras突变
A new methodology for the preparation of adressed DNA matrices is described. The process includes an electrochemically directed copolymerization of pyrrole and oligonucleotides bearing on their 5' end a pyrrole moiety introduced by phosphoramidite chemistry. The electro-controlled synthesis of the copolymer (polypyrrole) gives, in one step, a solid conducting film deposited on the surface of an electrode. The resulting polymer consists of pyrrole chains bearing covalently linked oligonucleotide. The polymer growth is limited to the electrode surface, so that it is possible to prepare a DNA matrix on a multiple electrode device by successive copolymerizations. A support bearing four oligonucleotides was used to detect three ras mutations on a synthetic DNA fragment