Conserved protein domains in a myosin heavy chain gene from Dictyostelium discoideum.
Conserved protein domains in a myosin heavy chain gene from Dictyostelium discoideum.
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盘基网柄菌的肌球蛋白重链基因中的保守蛋白结构域。
DOI：
10.1073/pnas.83.24.9433
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发表时间：
1986
影响因子：
11.1
通讯作者：
Spudich,JA
中科院分区：
文献类型：
--
作者：
Warrick,HM;DeLozanne,A;Leinwand,LA;Spudich,JA
通过对克隆基因的 DNA 序列分析确定盘基网柄菌的 2116 个氨基酸的肌球蛋白重链序列。基因产物可分为两个不同的区域：球状头部区域和长α螺旋杆状尾部。与线虫和哺乳动物肌肉肌球蛋白相比，头部区域的特定区域高度保守。这些区域可能反映了保守的功能和结构域。线虫和哺乳动物肌肉肌球蛋白头部区域存在的某些特征，以及被认为对肌球蛋白功能很重要的特征，在盘基网柄菌肌球蛋白序列中缺失。盘基网柄菌尾部区域的蛋白质序列相对于其他肌球蛋白保守性非常差，但显示出与肌肉肌球蛋白相似的周期性。这些周期性被认为在细丝形成中发挥作用。决定肌肉粗丝中所见的 14.3 nm 重复的 196 个残基重复单元、28 个残基电荷重复单元以及之前描述的线虫肌球蛋白的 1,4 疏水性重复单元都存在于盘基网柄菌肌球蛋白杆序列中，表明肌肉和盘基网柄菌肌球蛋白的丝结构必须相似。
The 2116-amino acid myosin heavy chain sequence from Dictyostelium discoideum was determined from DNA sequence analysis of the cloned gene. The gene product can be divided into two distinct regions, a globular head region and a long alpha-helical, rod-like tail. In comparisons with nematode and mammalian muscle myosins, specific areas of the head region are highly conserved. These areas presumably reflect conserved functional and structural domains. Certain features that are present in the head region of nematode and mammalian muscle myosins, and that have been assumed to be important for myosin function, are missing in the Dictyostelium myosin sequence. The protein sequence of the Dictyostelium tail region is very poorly conserved with respect to the other myosins but displays the periodicities similar to those of muscle myosins. These periodicities are believed to play a role in filament formation. The 196-residue repeating unit that determines the 14.3-nm repeat seen in muscle thick filaments, the 28-residue charge repeating unit, and the 1,4 hydrophobic repeat previously described for the nematode myosin are all present in the Dictyostelium myosin rod sequence, suggesting that the filament structures of muscle and Dictyostelium myosins must be similar.