Neferine alleviates P2X3 receptor in rat dorsal root ganglia mediated neuropathic pain
Neferine alleviates P2X3 receptor in rat dorsal root ganglia mediated neuropathic pain
复制标题
Neferine 减轻大鼠背根神经节 P2X3 受体介导的神经性疼痛
DOI：
10.1016/j.neures.2020.08.004
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发表时间：
2021-07-31
影响因子：
2.9
通讯作者：
Li, Guilin
中科院分区：
文献类型：
--
作者：
Dan, Yu;Guo, Hongmin;Li, Guilin
慢性神经性疼痛是由组织损伤或神经系统炎症引起的，其特征是对疼痛刺激敏感。P2X3受体在促进疼痛传递中起重要作用。莲子碱是从莲子胚中分离得到的双苄基异喹啉类生物碱，具有抗炎、抗氧化等药理作用。本研究探讨了碱是否能缓解与大鼠背根神经节（DRGs） P2X3受体相关的神经性疼痛。采用大鼠慢性收缩损伤（CCI）作为神经性疼痛模型。结果表明，P2X3受体在CCI大鼠DRGs中表达明显增加，机械异常性痛和热痛觉过敏也在CCI大鼠中增强。Neferine可显著降低CCI大鼠DRGs中P2X3受体和白细胞介素-1 - β的上调，抑制ERK1/2的磷酸化和活化，减轻神经性疼痛。因此，尼古丁通过下调P2X3受体的表达来缓解神经性疼痛。(c) 2020 Elsevier B.V.和日本神经科学学会。版权所有。
Chronic neuropathic pain is caused by tissue damage or nervous system inflammation and is characterized by sensitivity to painful stimuli. P2X3 receptors play an important role in facilitating pain transmission. Neferine is a bisbenzylisoquinline alkaloid isolated from seed embryos of lotus, which has anti-inflammatory and anti-oxidation pharmacological functions. The present research investigated whether neferine relieves neuropathic pain related to the P2X3 receptor in rat dorsal root ganglia (DRGs). Chronic contraction injury (CCI) in rats was used as a model for neuropathic pain. The results indicated that the expression of P2X3 receptor was significantly increased in the DRGs of CCI rats and that mechanical allodynia and thermal hyperalgesia were also enhanced in CCI rats. Neferine markedly lowered the upregulated P2X3 receptor and interleukin-1beta, inhibited the phosphorylation and activation of ERK1/2 in the DRGs of CCI rats, and relieved neuropathic pain. Therefore, neferine alleviates neuropathic pain by downregulating the expression of P2X3 receptor. (c) 2020 Elsevier B.V. and Japan Neuroscience Society. All rights reserved.