An evaluation of Brix refractometry instruments for measurement of colostrum quality in dairy cattle
An evaluation of Brix refractometry instruments for measurement of colostrum quality in dairy cattle
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DOI：
10.3168/jds.2009-2943
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发表时间：
2010-08-01
影响因子：
3.5
通讯作者：
Leslie, K. E.
中科院分区：
文献类型：
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作者：
Bielmann, V.;Gillan, J.;Leslie, K. E.
获得高质量的初乳是影响新生犊牛健康的重要因素。许多方法已被用于评估初乳中的IG浓度;然而，需要改进的、经验证的评估工具。本研究的目的是评价光学和数字Brix折射仪仪器与金标准放射免疫扩散试验实验室评估相比，用于测量初乳中IG浓度，并确定两种Brix折射仪仪器从新鲜和冷冻初乳样本中采集的IG测量值之间的相关性。本研究使用来自3个不同农场的288份初乳样品完成。得出的结论是，光学和数字白利糖度折光仪是高度相关的新鲜和冷冻样品（r = 0.98和r = 0.97，分别）。新鲜和冷冻样品的折射仪仪器和金标准径向免疫扩散测定之间的相关性被确定为非常相似，相关系数在0.71和0.74之间。这两种仪器都表现出优异的测试特性，表明22%的白利糖度分数的适当的临界点，用于鉴定质量良好的初乳。
Acquisition of high quality colostrum is an important factor influencing neonatal calf health. Many methods have been used to assess the Ig concentration of colostrum; however, improved, validated evaluation tools are needed. The aims of this study were to evaluate both optical and digital Brix refractometer instruments for the measurement of Ig concentration of colostrum as compared with the gold standard radial immunodiffusion assay laboratory assessment and to determine the correlation between Ig measurements taken from fresh and frozen colostrum samples for both Brix refractometer instruments. This research was completed using 288 colostrum samples from 3 different farms. It was concluded that the optical and digital Brix refractometers were highly correlated for both fresh and frozen samples (r = 0.98 and r = 0.97, respectively). Correlation between both refractometer instruments for fresh and frozen samples and the gold standard radial immunodiffusion assay were determined to be very similar, with a correlation coefficient between 0.71 and 0.74. Both instruments exhibited excellent test characteristics, indicating an appropriate cut-off point of 22% Brix score for the identification of good quality colostrum.